CORE--ANIMAL BARRIER FACILITY

核心——动物屏障设施

• 批准号: 6396727
• 负责人: JEFFREY A BLUESTONE
• 金额: --
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6396727
• 关键词: animal colony; biomedical facility; germ free condition; laboratory mouse

The goal of the University of Chicago Cancer Research Center (UCCRC) Animal Barrier Facilities is to provide Cancer Center members the ability to conduct studies with rodents free of intercurrent infection with adventitious murine pathogens. The Barriers are supported by the facilities, programs, and staff of the University's Animal Resources Center (ARC). The ARC's Comparative Diagnostic Laboratory(CDL) provides expertise in rodent serology, microbiology, parasitology, and hematology. All biological materials introduced into rodents maintained in these facilities are evaluated by the CDL for contamination with adventitious viruses and mycoplasma by MAP testing. The ARC conducts an extensive Murine Health Monitoring Program to confirm that the pathogen-free status of the barrier colonies are maintained. The program is designed to detect variance in health status at specific time intervals. The program employs sentinel animals monitored monthly, serum banking, evaluation of "index" cases and close communication with investigative staff who may note unexplained variances in their experiments. Additionally, a quarantine program which includes extensive diagnostic evaluation is instituted for all rodents imported from colonies obtained from non-commercial vendors. Quarantine is essential to minimize the likelihood of introducing unwanted murine pathogens into barrier colonies. The rodent barrier facilities serving the UCCRC are located on the top floor of the Franklin McLean Institute (FMI) and in the basement of Biological Sciences Learning Center (BSLC). They are essential components of the Cancer Research Center, providing approximately 7,925 net square feet of animal holding and procedure space. The facilities contain sixteen animal holding rooms, seven of which are equipped with three Illinois cubicles. In addition to animal holding rooms, the barriers are equipped with laboratory and procedure space. Isolator type caging systems are employed throughout the barriers. All caging materials and feed are autoclaved prior to use. Animals are transferred within a separate hood or within a cubicle by animal care specialists, using chemically disinfected forceps between cage transfers. A maximum of four or five mice, dependent on weight, are permitted per cage. A bar coded cage card provides encrypted information including the strain or stock, source, and investigator. Cage cards are routinely scanned for information which is downloaded into the ARC database that is used to maintain accurate and detailed census information.

芝加哥大学癌症研究中心的目标 动物屏障设施是为癌症中心成员提供的能力 在没有混合感染的啮齿动物身上进行研究 不确定的老鼠病原体。这些障碍由 大学动物资源的设施、项目和工作人员 中心(ARC)。ARC的比较诊断实验室(CDL)提供 在啮齿动物血清学、微生物学、寄生虫学和血液学方面的专业知识。 所有引入啮齿动物体内的生物材料都在这些 美国疾病控制与预防中心对设施进行评估，以确定是否存在外来物质的污染 MAP检测病毒和支原体。ARC进行了广泛的 小鼠健康监测程序确认无病原体状态 屏障菌落的数量得以维持。该程序旨在检测 在特定时间间隔内健康状态的变化。该计划雇用了 哨兵动物每月监测，血清库，评估“指标” 案件并与调查人员密切沟通，他们可能会注意到 在他们的实验中有无法解释的差异。另外，一个检疫 制定了包括广泛诊断评估的计划 从非商业供应商处获得的从殖民地进口的所有啮齿动物。 隔离对于最大限度地减少传入的可能性至关重要 不想要的老鼠病原体变成屏障菌落。 UCCRC的鼠患屏障设施位于顶部 富兰克林·麦克莱恩研究所(FMI)的楼层和 生物科学学习中心(BSLC)它们是必不可少的组件 癌症研究中心，提供约7925平方米的净面积 动物的脚和程序空间。这些设施包括 16个动物休息室，其中7个配有3个 伊利诺伊州的隔间。除了动物拘留室，障碍物还有 配有实验室和程序空间。隔离器式保持架 在所有障碍中都采用了系统。所有的笼子材料和 饲料在使用前要进行高压灭菌。动物在一个 单独的引擎盖或由动物护理专家在隔间内使用 在转笼之间用化学消毒的钳子。最多四个 或者，根据体重，每个笼子允许五只小鼠。条形码 笼卡提供包括菌株或股票的加密信息， 线人和调查员。笼卡通常被扫描以查找 下载到ARC数据库的信息，用于 维护准确和详细的人口普查信息。