CORE--SYNTHETIC ANTIGEN LABORATORY

核心--合成抗原实验室

• 批准号: 6217265
• 负责人: RALPH BERNARD ARLINGHAUS
• 金额: $ 33.23万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6217265
• 关键词: antigens; biomedical facility; high performance liquid chromatography; peptide chemical synthesis; protein purification; protein sequence; synthetic peptide

The Synthetic Antigen Facility provides synthetic peptides in a highly purified state to faculty members of the University of Texas M.D. Anderson Cancer Center. This facility is located in room B8.4806 (544 feet/2) and is equipped with a special enhanced exhaust system for handling HF. Facility staff members provide consultation to the research staff regarding synthetic peptides. The institution is provide new peptide synthesizers and high-performance liquid chromatography (HPLC) equipment that will double the facility's current capacity and decrease its turnaround time. Funds from the facility's charge-back account cover the cost of materials and the salary for one research investigator. An oversight committee exists consisting of John McMurray, P.h.D., Benoit deCrombrugghe, M.D., and Ralph Arlinghaus, P.h.D. The major functions of this committee are to review the operation of the facility, make suggestions for improvement, set priorities, and ensure the satisfaction of the users. Ninety-three of the 122 peptides made last year (7/1/96 to 6/30/97) were produced for peer-funded investigators, reflecting the facility's goal to provide high-quality reagents to the most competitive and productive faculty members. These peptides were provided to a total of 21 users, 14 of whom were peer funded. Since the last competitive renewal, 936 peptides were synthesized, 612 of which were for peer-funded users (7/1/91 to 6/30/97). In general the purity of the peptides provided to users was routinely 90% or higher,a nd some peptides were supplied at a purity greater that 95%. One example of the type of peptides supplied this past year is a 42-amino-acid peptide synthesized for Dr. Larry Etkin (Department of Molecular Genetics). The sequence of this peptide was derived from a unique zinc-finger domain discovered by Dr. Etkin. Research studies involving this peptide by a physical chemistry group in London, England, established the structure of this domain. The key factors were the large amount of peptide supplied and the low cost compared with costs of outside competitors. Another examine is a phosphotyrosine peptide supplied to Dr. Gordon Mills (Department of Molecular Oncology). This synthesis required an alternative method of phosphorylation from that used by many in the field. Normally, the phosphate is added to the Y residue after synthesis, but because of the presence of oxidation-sensitive residues (e.g., two methionines), a protected, phosphorylated amino-acid derivative was used for the synthesis phase (tBOC dimethylphosphotyrosine). The phosphotyrosine peptide was provided at 98% purity.

合成抗原设施提供合成肽， 得克萨斯大学医学博士的教职员工。安德森 癌症中心该设施位于B8.4806室（544英尺/2）， 配备了用于处理HF的特殊增强排气系统。 设施工作人员为研究人员提供咨询 关于合成肽。该机构正在提供新的肽 合成器和高效液相色谱（HPLC）设备 这将使该设施目前的容量增加一倍， 周转时间该设施的收费帐户的资金涵盖 材料费和一名研究员的工资。一个 监督委员会由约翰·麦克默里博士组成，Benoit deCrombrugghe，医学博士，拉尔夫·阿林豪斯博士的主要功能 该委员会将审查该设施的运作， 提出改进建议，确定优先事项，并确保满意度 的用户。去年制造的122种肽中有93种（1996年7月1日至 1997年6月30日）为同行供资的调查员编制， 设施的目标是提供高质量的试剂，以最具竞争力的 和富有成效的教职员工。这些肽被提供给总共 21个用户，其中14个是同行资助的。自从上次竞争性续约以来， 合成了936个肽，其中612个是为同行资助的用户 （1991年7月1日至1997年6月30日）。一般来说，提供给 使用者通常为90%或更高，并且一些肽以 纯度大于95%。提供这种肽的一个例子是， 去年是一个42个氨基酸的肽合成博士拉里埃特金 （分子遗传学系）。该肽的序列为 由埃特金博士发现的独特锌指结构域衍生而来。研究 伦敦的一个物理化学小组对这种肽进行了研究， 英国，建立了这个领域的结构。关键因素是 肽的供应量大，与成本相比， 外部竞争者。另一种检查是磷酸酪氨酸肽 提供给Gordon米尔斯博士（分子肿瘤科）。这 合成需要从所用的磷酸化方法中选择一种方法， 很多人在外地。通常，磷酸盐被添加到Y残基中 合成后，但由于氧化敏感的存在， 残基（例如，两个蛋氨酸），一种受保护的磷酸化氨基酸 衍生物用于合成阶段（tBOC 二甲基磷酸酪氨酸）。磷酸酪氨酸肽的提供率为98%。 纯度