CORE--ANALYTICAL CHEMISTRY/MOLECULAR BIOLOGY

核心--分析化学/分子生物学

• 批准号: 6296590
• 负责人: JOHN D. TESSARI
• 金额: $ 14.74万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2001-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6296590
• 关键词: analytical chemistry; biomedical facility; computer program /software; gas chromatography; gel electrophoresis; industrial waste; microorganism culture; molecular biology; nucleic acid sequence; polymerase chain reaction; single strand conformation polymorphism

This core will provide analytical chemistry and molecular biological support to the 9 individual projects for this Program Project "Biological and Chemical Interactions of Chemical Mixtures. "The specific aims of this core are 1) to prepare and analyze by spectrophotometry and gas chromatography the chemical mixture solutions as needed by the individual projects; 2) to provide analytical analyses in tissue, water and culture media as needed by the individual projects; 3) to provide access to facilities and equipment to set up and use PCR reactions and analyze the products of those reactions. This includes a variety of PCR based techniques including RAPD-PCR, RT-PCR, differential display and cycle sequencing; 4) to provide the expertise and software for analysis of RAPD- PCR population genetics data; 5) to provide facilities to set up, run, score and analyze sequencing gels; 6) to provide facilities to set up, run and silver stain SSCP gels. A major function of this core will be the integration of analytical chemistry/molecular biology support for the 9 interdisciplinary research projects. The core has the ability and the expertise to provide immediate support and an effective response to all the needs of the Program Project and interrelated research in Biomedical/Ecological/Epidemiological areas.

这个核心将提供分析化学和分子生物学 支持本计划项目的9个单独项目“生物 和化学混合物的化学相互作用 “这一行动的具体目标是 核心是1）通过分光光度法和气相色谱法制备和分析 色谱分析化学混合物的解决方案，根据个人的需要 项目; 2）提供组织、水和培养物的分析 （3）根据具体项目的需要， 建立和使用PCR反应并分析 这些反应的产物。 这包括各种基于PCR的 RAPD-PCR、RT-PCR、差异显示和循环等技术 测序; 4）提供RAPD分析的专业知识和软件- PCR群体遗传学数据; 5）提供设施，以建立，运行， 评分和分析测序凝胶; 6）提供设施，以建立，运行 和银染SSCP凝胶。 该核心的一个主要功能是整合分析 化学/分子生物学支持9个跨学科研究 项目 核心有能力和专业知识， 支持和有效响应计划项目的所有需求 生物医学/生态学/流行病学领域的相关研究。