CORE--ANALYTICAL CHEMISTRY/MOLECULAR BIOLOGY

核心--分析化学/分子生物学

• 批准号: 6340929
• 负责人: JOHN D. TESSARI
• 金额: $ 14.74万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2001-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6340929
• 关键词: analytical chemistry; biomedical facility; computer program /software; gas chromatography; gel electrophoresis; industrial waste; microorganism culture; molecular biology; nucleic acid sequence; polymerase chain reaction; single strand conformation polymorphism

This core will provide analytical chemistry and molecular biological support to the 9 individual projects for this Program Project "Biological and Chemical Interactions of Chemical Mixtures. "The specific aims of this core are 1) to prepare and analyze by spectrophotometry and gas chromatography the chemical mixture solutions as needed by the individual projects; 2) to provide analytical analyses in tissue, water and culture media as needed by the individual projects; 3) to provide access to facilities and equipment to set up and use PCR reactions and analyze the products of those reactions. This includes a variety of PCR based techniques including RAPD-PCR, RT-PCR, differential display and cycle sequencing; 4) to provide the expertise and software for analysis of RAPD- PCR population genetics data; 5) to provide facilities to set up, run, score and analyze sequencing gels; 6) to provide facilities to set up, run and silver stain SSCP gels. A major function of this core will be the integration of analytical chemistry/molecular biology support for the 9 interdisciplinary research projects. The core has the ability and the expertise to provide immediate support and an effective response to all the needs of the Program Project and interrelated research in Biomedical/Ecological/Epidemiological areas.

这个核心将提供分析化学和分子生物学 支持本计划项目“生物”的9个单独项目 和化学混合物的化学相互作用。“这一行动的具体目的是 岩心：1)用分光光度和气体分析方法制备和分析 根据个人需要对化学混合物溶液进行层析 项目；2)提供组织、水和培养方面的分析 根据个别项目的需要提供媒体；3)提供访问 建立和使用聚合酶链式反应和分析 这些反应的产物。这包括各种基于PCR的 包括随机扩增、逆转录聚合酶链式反应、差异显示和循环显示等技术 测序；4)为随机扩增多态性DNA的分析提供专业技术和软件 聚合酶链式反应群体遗传学数据；5)提供设施以设置、运行、 对测序凝胶进行评分和分析；6)提供设施以设置、运行 银染SSCP凝胶。 这个核心的一个主要功能将是集成分析 为9项跨学科研究提供化学/分子生物学支持 项目。核心有能力和专业知识立即提供 支持并有效响应计划项目的所有需求 以及生物医学/生态/流行病学领域的相关研究。