CORE--MORPHOLOGY

这个核心是一个中央形态设施，为 项目中的调查员。所提供的服务包括 常规光学显微镜处理，组织切片分析，以及 特殊污渍。此外，如果研究人员希望， 透射电子显微镜（TEM），免疫荧光， 免疫组织化学和形态测定分析服务。 最后，该核心提供数字图像分析和图形 为调查人员提供支持。在过去，这个核心 预计需求将继续增长， 增加例如，在过去的一年中， 为程序项目的各个部分处理。在这个总数中 有1640个样品提交进行光学显微镜分析，305个样品用于 免疫组织化学和/或免疫荧光研究和181个样本， 透射电镜 我们预计，将有大量使用这一核心的 未来几年的调查。例如，在第一、二、三、四节中， 和IV光学显微镜将常规用于监测 各种模型中的肺损伤。此外，体外研究表明， 将在所有切片中进行分离细胞和组织培养单层 使用这种技术。TEM研究也将广泛用于这一领域。 项目的所有部分都使用了这种技术。 主要利用TEM对组织改变进行形态测定分析， 在第II、III和IV部分的选定组织上进行。形态测定 需要进行分析以精确地定量肺损伤 参数为肺泡和内皮细胞损伤百分比， 白细胞和出血的定量常规评价， 法此外，第二节将使用形态测量法评估尺寸 以及肺内肉芽肿的分布情况。冰冻切片分析 组织和细胞，特别是免疫组织化学研究， 调查人员目前大量使用， 目前的提案。免疫组织化学是用于 评估体内和体外粘附分子和细胞因子的上调， 体外并将被计划项目的所有部分使用。 选定的免疫荧光研究也将使用冷冻的 部分以评价免疫复合物诱导的肺损伤和 第1节中的主要干预性研究。最后，核心设施 提供数字图像分析和图形服务，包括彩色数字 激光打印供研究人员使用的海报和出版物 程序项目。

This core is a central morphology facility providing support for the investigators in the program project. The services provided include routine light microscopy processing, analysis of tissue sections, and special stains. In addition, where desired by the investigators, transmission electron microscopy (TEM), immunofluorescence, immunohistochemistry, and morphometric analysis services are available. Finally, this core provides digital image analysis and graphic illustration support for the investigators. In the past this core has received heavy usage and it is anticipated that demand will continue to increase. For example, during this past year over 2100 samples were processed for the sections of the program project. Of this total there were 1640 samples submitted for light microscopy analysis, 305 samples for immunohistochemistry and/or immunofluorescence studies and 181 samples for TEM. We anticipate that there will be heavy usage of this core by the investigators in the coming years. For example, in sections I, II, III, and IV light microscopy will be routinely used to monitor the intensity of the lung injury in various models. In addition, in vitro studies of isolated cells and tissue culture monolayers will be done in all sections using this technique. TEM studies will also be used extensively in this program project with all of the sections utilizing this technique. Morphometric analyses of tissue alterations utilizing primarily TEM will be done on selected tissues for sections II, III, and IV. Morphometric analysis is required to precisely quantitate injury to the lung with such parameters as percent alveolar and endothelial cell injury and quantitation of leukocytes and hemorrhage evaluated routinely by this method. In addition section II will use morphometry to evaluate the size and distribution of the granulomas in the lung. Frozen sections analysis of tissues and cells, particularly for immunohistochemistry studies, is heavily utilized currently by investigators and this will continue in the current proposal. Immunohistochemistry is the primary technique used for evaluating adhesion molecule and cytokine up-regulation in vivo and in vitro and will be utilized by all sections of the program project. Selected immunofluorescence studies will also be done utilizing frozen sections to evaluate immune complex induced lung injury and the effect of interventional studies primary in section 1. Finally, the core facility offers digital image analysis and graphic services including color digital laser printing for posters and publications for investigators in the program project.