CORE--Morphology Core

该核心是一个中央形态学设施，它为参与该计划项目的个人调查员提供支持。提供的服务包括常规光学显微镜（H&E）处理，组织切片分析和特殊染色。此外，如果研究人员需要，还可以提供超微结构、免疫荧光、免疫组织化学和形态分析服务。最后，该核心为研究人员提供了重要的数字图像分析和图形说明支持。在过去，这种核心得到了大量的使用，预计需求将继续增加。例如，在过去的一年里，超过1276个样本被处理，以支持在这个项目中进行的研究。其中，有975个样本提交给光学显微镜分析（H&E）， 931个空白被切割用于免疫组织化学和/或免疫荧光，58个被处理用于特殊染色。此外，去年进行了124个样品的TEM处理。我们预计该核心将继续在项目中发挥积极作用，研究人员将广泛使用形态学核心的服务。例如，每个具体项目将常规利用光学显微镜监测各种实验模型中肺损伤的强度和细胞结构。组织切片的形态计量学分析将用于量化肺损伤，测量各种参数，包括肺肉芽肿的发展大小，肺泡和内皮细胞损伤的百分比以及肺泡间隙白细胞和出血的定量。在体内标本和体外培养细胞的具体研究中，将通过免疫组织化学分析进行评估。这些组织和细胞的冷冻切片将被评估各种细胞因子的蛋白质，这些细胞因子可能被表达并作为肺部炎症进展的机制。这是本项目要求的唯一技术核心，也是该项目的主要支持机制

This Core is a central morphology facility, which provides support for the individual investigators who ocmprise this Program Project. The services provided include routine light microscopy (H&E) processing, analysis of tissue sections, and special stains. In addition, when desired by the investigators, ultrastructural, immunofluorescence, immunohistochemistry, and morphometic analysis services are available. Finally, this core provides important digital image analysis and graphic illustration support for the investigators. In the past this core has received heavy usage and it is anticipated that demand will continue to increase. For example, during the past year over 1276 samples were processed to support the studies carried out in this Program Project. Of this total, there were 975 samples submitted for light microscopy analysis (H&E), 931 blanks were cut for imunohistochemistry and/or immunoflurescence, and 58 were processed for special stains. In addition last year 124 samples were processed for TEM. We anticipate that this Core will continue to play an active role in the Program Project and the investigators will extensively use the services of the morphology core. For example, each of the specific projects will routinely utilize light microscopy to monitor the intensity and cellularity of the lung injury in the various experimental models. Morphometric analysis of tissue sections will be used to quantitate lung injury with a variety of parameters measured, including the developing size of lung granulomas, percent alveolar and endothelial cell injury and quantitation of leukocytes and hemorrhage in the alveolar space. In specific studies of in vivo specimens and in vitro cultured cells will be assessed by immunohistochemical analysis. Frozen sections of these tissues and cells will be assessed for the protein of a variety of cytokines that are likely to be expressed and serve as mechanisms for the progression of lung inflammation. This is the only technical Core requested for this Program Project and is the main supporting mechanism for the morphology associated with the various sections. The activity of this Core is of critical importance to the success of the Program Project. This Core also offers digital image analysis and graphic services, including color digital laser printing for posters and publications for the investigators and their team of researchers associated with this Program Project.