CIRCULATING NITRIC OXIDE CONCENTRATION IN AGING RHESUS MACAQUES

目的探讨一氧化氮（NO）在恒河猴衰老过程中的作用 猕猴模型。 结果NO浓度有降低的趋势 在年龄较大的绝经后猴子中发现， NO的浓度似乎不受年龄或长期饮食的影响 限制雄性恒河猴。 一氧化氮（NO）是 被认为在衰老过程中发挥作用， 在更年期低。 饮食限制（DR）延缓衰老， 增加啮齿类动物的寿命，最近的研究表明， 对恒河猴和食蟹猴的健康有益。 在 为了探讨NO在非人类衰老中的可能作用， 在灵长类动物模型中，我们测量了 绝经前（n=9;年龄12.6 ± 10.3岁[平均1 SEM]）和绝经后 （n=10;年龄26.8 ± 10.4岁）雌性恒河猴，年轻成年（n=9; 年龄11.9 ± 10.5岁）和老年（n=10;年龄26.7 ± 11.0岁）雄性恒河猴 猴子和雄性恒河猴（16-22岁）， 30%DR 8年（n=12）及其配对对照组 自由进食（n=12）。 绝经前的猴子 血清NO浓度高于绝经后猴37.89 ± 12.79 5 M vs. 31.2312.66 5M，p=0.099。 相比之下，年轻的成年男性 猕猴的血清NO浓度与老年男性没有差异 分别为37.8414.39 5 M和37.1714.84 5 M。 此外，血浆NO值 对照组和限制性雄性猴之间无差异34.0814.17 5 M与33.6512.88 5 M。 略低NO的趋势 老年绝经后猴子的浓度值得进一步研究。 调查 另一方面，循环中的NO水平不 在雄性恒河猴中，似乎受年龄或长期DR的影响 猴子 这些结果并不排除一个重要的作用， 局部的，即，组织特异性，对无数 在绝经过渡期发生的生理变化， 作为未来方向博士的结果，我们计划进一步 探讨循环NO与肝硬化的关系及意义。 与绝经过渡期和饮食限制的关系。 关键 饮食限制，衰老，更年期基金NIH PO 1 AG 11915

OBJECTIVE To explore the possible role of NO in aging in a rhesus macaque model. RESULTS A trend for somewhat lower NO concentrations was found in older, postmenopausal monkeys, while circulating levels of NO did not seem to be influenced by age or long-term dietary restriction in male rhesus monkeys. DISCUSSION Nitric oxide (NO) has been proposed to play a role in the aging process and to be relatively low during menopause. Dietary restriction (DR) retards aging and increases lifespan in rodents, and it has recently been shown to have beneficial effects on health in rhesus and cynomolgus macaques. In order to explore the possible role of NO in aging in this nonhuman primate model, we measured circulating levels of NO in group of premenopausal (n=9; aged 12.610.3 years [mean1SEM]) and postmenopausal (n=10; aged 26.810.4 years) female rhesus monkeys, young adult (n=9; aged 11.910.5 years) and old (n=10; aged 26.711.0 years) male rhesus monkeys, and in male rhesus monkeys (16-22 years old) that had undergone 30% DR for 8 years (n=12) and their matched control group fed ad libitum (n=12). Premenopausal monkeys had marginally higher serum NO concentration than postmenopausal monkeys 37.8912.79 5M vs. 31.2312.66 5M, respectively, p=0.099. In contrast, young adult male macaques did not differ from older males in serum NO concentration 37.8414.39 5M vs. 37.1714.84 5M, respectively. Also, plasma NO values did not differ between control and restricted male monkeys 34.0814.17 5M vs. 33.6512.88 5M, respectively. The trend for somewhat lower NO concentrations in the older, postmenopausal monkeys merits further investigation. On the other hand, circulating levels of NO do not seem to be influenced by age or by long-term DR in male rhesus monkeys. These results do not preclude an important role for localized, i.e., tissue specific, NO influences on the myriad physiological changes that occur during the menopausal transition and as a consequence of DR. FUTURE DIRECTIONS We plan to further investigate the relationship and significance of circulating NO in relation to the menopausal transition and dietary restriction. KEY WORDS Dietary restriction, aging, menopause FUNDING NIH PO1 AG11915