NEUROENDOCRINE REGULATION OF REPRODUCTIVE SUCCESS IN FEMALE MARMOSETS

目的探讨黄体生成素对脑垂体的调节作用。 促黄体生成素分泌在神经内分泌机制中的作用 抑制雌性绒猴的排卵。结果 本研究的目的是开始探索脑垂体水平。 社会调节生育力背后的神经内分泌机制 这种灵长类动物。实验1同时测定GnRH(通过PPP)和 血浆促黄体生成素(黄体生成素，通过留置颈静脉导管) 非从属卵巢摘除(OVX)女性(n=5)，每10分钟治疗一次 至12小时。尽管促性腺激素释放激素(1.12脉冲/小时+0.09)和黄体生成素(0.96脉冲/小时 +0.09)脉冲频率相似(p=0.4)，脉冲峰值不同 在时间上协调一致。在实验2中，非从属的OVX雌性 (n=3)分别接受2次静脉注射促性腺激素释放激素(200 ng，25g)，同时 每隔5分钟采血一次。黄体生成素反应高峰潜伏期 GnRH刺激延迟29.2min±3.5min。总的来说，这些结果 表明在一种适应极端心理社会调节的灵长类动物中 在生殖方面，(1)垂体促黄体生成素的释放不是一个可靠的暂时性 下丘脑GnRH释放的生物标志物及其机制 生育力的社会调节可能在脑下垂体起调节作用 水平。我们计划调查的未来方向具体 脑垂体促性腺激素反应的神经内分泌改变 雌性绒猴下丘脑GnRH的释放 增加我们对这一潜在的新法规的理解 女性的生殖功能。关键词促性腺激素释放 激素，不孕不育，脑下垂体，促黄体激素，绒猴资金 NIH RR0167，NIH MH11417

OBJECTIVE To determine the role of pituitary-level regulation of LH secretion in the neuroendocrine mechanisms mediating social suppression of ovulation in subordinate female marmosets. RESULTS The purpose of the present study was to begin exploring pituitary-level neuroendocrine mechanisms underlying socially-modulated fertility in this primate. Experiment 1 simultaneously measured GnRH (via PPP) and plasma luteinizing hormone (LH, via indwelling jugular catheter) in non-subordinate ovariectomized (OVX) females (n=5) every 10min for up to 12hrs. Although GnRH (1.12 pulse/hr + 0.09) and LH (0.96 pulse/hr + 0.09) pulse frequencies were similar (p=0.4), pulse peaks were not temporally concordant. In Experiment 2, non-subordinate OVX females (n=3) each received 2 intravenous GnRH challenges (200ng, 25g) while plasma LH was sampled every 5min. The latency of peak LH response to GnRH stimulation was delayed by 29.2min + 3.5. Overall, these results suggest that in a primate adapted for extreme psychosocial regulation of reproduction, (1) pituitary LH release is not a reliable temporal biomarker for hypothalamic GnRH release and (2) mechanisms underlying social modulation o f fertility may be mediated at the pituitary level. FUTURE DIRECTIONS We plan to investigate specific neuroendocrine alteration of pituitary gonadotropic response to hypothalamic GnRH release in subordinate female marmosets in order to increase our understanding of this potentially novel regulation of female reproductive function. KEY WORDS gonadotropin-releasing hormone, infertility, pituitary, luteinizing hormone, marmoset FUNDING NIH RR0167, NIH MH11417