NEUROENDOCRINE REGULATION OF REPRODUCTIVE SUCCESS IN FEMALE MARMOSETS

目的探讨垂体水平对脑缺血再灌注的调节作用。 促黄体生成素分泌在介导社会功能的神经内分泌机制中的作用 抑制下级雌性绒猴的排卵。 结果 本研究的目的是开始探索垂体水平的 神经内分泌机制潜在的社会调节生育能力， 这个灵长类动物 实验1同时测量GnRH（通过PPP）和 血浆黄体生成素（LH，通过颈静脉导管留置）， 非从属性卵巢切除（OVX）雌性动物（n=5），每10分钟一次，直至 到12小时。 虽然GnRH（1.12脉冲/小时± 0.09）和LH（0.96脉冲/小时 + 0.09）脉冲频率相似（p=0.4），脉冲峰值不相似 时间上一致的 在实验2中， （n=3）分别接受2次静脉注射GnRH激发（200 ng，25 g）， 每5分钟采集一次血浆LH。 LH峰值反应的潜伏期 GnRH刺激延迟29.2min ± 3.5。 总的来说，这些结果 表明在适应极端社会心理调节的灵长类动物中 （1）垂体LH释放不是一个可靠的时间 下丘脑GnRH释放的生物标志物和（2）潜在机制 生育力的社会调节可能在垂体介导 水平 未来的方向我们计划调查具体的 垂体促性腺激素反应的神经内分泌改变 次级雌性绒猴下丘脑GnRH释放， 增加我们对这种潜在的新的调节的理解， 女性生殖功能 关键词促性腺激素释放 激素，不孕症，垂体，促黄体激素，绒猴基金 NIH RR0167，NIH MH11417

OBJECTIVE To determine the role of pituitary-level regulation of LH secretion in the neuroendocrine mechanisms mediating social suppression of ovulation in subordinate female marmosets. RESULTS The purpose of the present study was to begin exploring pituitary-level neuroendocrine mechanisms underlying socially-modulated fertility in this primate. Experiment 1 simultaneously measured GnRH (via PPP) and plasma luteinizing hormone (LH, via indwelling jugular catheter) in non-subordinate ovariectomized (OVX) females (n=5) every 10min for up to 12hrs. Although GnRH (1.12 pulse/hr + 0.09) and LH (0.96 pulse/hr + 0.09) pulse frequencies were similar (p=0.4), pulse peaks were not temporally concordant. In Experiment 2, non-subordinate OVX females (n=3) each received 2 intravenous GnRH challenges (200ng, 25g) while plasma LH was sampled every 5min. The latency of peak LH response to GnRH stimulation was delayed by 29.2min + 3.5. Overall, these results suggest that in a primate adapted for extreme psychosocial regulation of reproduction, (1) pituitary LH release is not a reliable temporal biomarker for hypothalamic GnRH release and (2) mechanisms underlying social modulation o f fertility may be mediated at the pituitary level. FUTURE DIRECTIONS We plan to investigate specific neuroendocrine alteration of pituitary gonadotropic response to hypothalamic GnRH release in subordinate female marmosets in order to increase our understanding of this potentially novel regulation of female reproductive function. KEY WORDS gonadotropin-releasing hormone, infertility, pituitary, luteinizing hormone, marmoset FUNDING NIH RR0167, NIH MH11417