INTESTINAL CYTOKINE & T CELL HOMEOSTASIS IN SIV INFECTION

肠相关淋巴组织（GALT）是一种重要的 早期病毒复制和传播位点与严重的CD 4 + T 细胞耗竭 这些变化没有充分反映在 外周血或淋巴结。 GALT将在确定 SIV相关的CD 4 + T细胞耗竭机制。 目标本 本研究是为了检查病毒的动力学， GALT中的复制和CD 4 + T细胞耗竭， 在SIV感染过程中， 恒河猴 结果病毒复制和严重的CD 4 + T细胞 恒河猴SIV感染时GALT出现耗竭 猕猴 这伴随着肠道CD 8 + T细胞的增加。 细胞百分比和Th 1型表达的增加潜力 细胞因子 这些变化可能反映了T细胞稳态的改变， GALT在SIV相关肠病中起重要作用， 病毒致病机制 未来的研究将集中在 确定SIV相关的CD 4 + T细胞耗竭的机制， GALT。 SIV感染猕猴的抗逆转录病毒治疗研究 将进行以确定CD 4 + T细胞的机制， GALT中的再增殖和病毒抑制。 关键词肠道相关， 淋巴组织，病毒复制，SIV基金NIH Grant AI 043274 Smit-McBride，Z.，Mattapallil，J.，McChesney，M.， Ferrick，D.和Dandekar，S. Journal of Virology 72：6646-6656，1998. Mattapallil，J，Smit-McBride Z，McChesney，M和Dandekar，S.杂志 of Virology 72：6421-6429，1998. Smit-McBride，Z，Mattapallil，J.， Villinger，F.，Ansari A，Dandekar，S.灵长类医学杂志 27：129-140，1998.

Significance Gut-associated lymphoid tissue (GALT) is an important site for early viral replication and dissemination and severe CD4+ T cell depletion. These changes are not adequately reflected in peripheral blood or lymph nodes. GALT will be valuable in determining mechanisms of SIV-associated CD4+ T cell depletion. Objectives The present study was performed to examine the kinetics of viral replication and CD4+ T cell depletion in GALT in comparison with peripheral blood and lymph nodes during the course of SIV infection in rhesus macaques. Results Viral replication and severe CD4+ T cell depletion occurred in GALT during primary SIV infection in rhesus macaques. This was accompanied by an increase in intestinal CD8+ T cell percentages and increased potential of expression of Th1 type cytokines. These changes could reflect altered T cell homeostasis in GALT and play an important role in SIV-associated enteropathy and viral pathogenesis. Future Directions Future studies will focus on determining the mechanisms of SIV-associated CD4+ T cell depletion in GALT. Studies of anti-retroviral therapy of SIV-infected macaques will be performed to determine the mechanisms of CD4+ T cell repopulation and viral suppression in GALT. KEY WORDS gut-associated, lymphoid tissue, viral replication, SIV FUNDING NIH Grant AI043274 PUBLICATIONS Smit-McBride, Z., Mattapallil, J., McChesney, M., Ferrick, D. and Dandekar, S. Journal of Virology 72:6646-6656, 1998. Mattapallil, J, Smit-McBride Z, McChesney, M, and Dandekar, S. Journal of Virology 72:6421-6429, 1998. Smit-McBride, Z, Mattapallil, J., Villinger, F., Ansari A, Dandekar, S. Jounal of Medical Primatology 27:129-140, 1998.