DAUNORUBICIN DPSH GENE:STARTER UNIT & CYCLIZ PATTERN:TYPE II POLYKETIDE SYNTHASE
柔红霉素 DPSH 基因:起始单元
基本信息
- 批准号:6120949
- 负责人:
- 金额:$ 0.74万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1999
- 资助国家:美国
- 起止时间:1999-03-01 至 2000-02-29
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Periplasmic transport systems of Gram-negative bacterial are
responsible for transporting a wide variety of substances, such as
sugars, amino acids, peptides, anions, and other nutrients, across the
cytoplasmic membrane. The bacterial transport systems are composed of
a ligand-specific binding protein and a membrane-bound protein
complex. The binding proteins are located in the periplasmic space,
and act as receptors for a specific ligand. The glutamine permease
system provides a good model for the NMR study of the essential
processes of the periplasmic binding protein-dependent membrane
transport. The binding protein of the system, glutamine-binding
protein (GlnBP), is a monomeric basic protein, with 226 amino-acid
residues and a molecular weight of 24,935 Daltons. It binds
L-glutamine with a molar ratio of 1:1 at neutral pH. In order to
understand the high specificity and affinity of GlnBP for its ligand
as well as the detailed mechanisms of ligand recognition and binding,
a solution structure of GlnBP-Gln complex is necessary. We have been
working on the backbone NMR resonance assignments of GlnBP and have
assigned about 95% of the residues. Now we will start working on the
sidechain assignments and structure determination. H(CCO)NH and 4D
NOESY experiment are expected to be run for this purpose.
革兰氏阴性菌的周质转运系统是
负责运输各种物质,例如
糖、氨基酸、肽、阴离子和其他营养素,
细胞质膜 细菌转运系统由以下组成:
配体特异性结合蛋白和膜结合蛋白
复杂. 结合蛋白位于周质间隙中,
并作为特定配体的受体。 谷氨酰胺通透酶
系统为核磁共振研究提供了一个很好的模型
周质结合蛋白依赖膜的突起
运输 该系统的结合蛋白,谷氨酰胺结合
GlnBP是一种由226个氨基酸组成的碱性蛋白质
残基和24,935道尔顿的分子量。 其结合
L-谷氨酰胺,在中性pH下摩尔比为1:1。
了解GlnBP对其配体的高特异性和亲和力
以及配体识别和结合的详细机制,
GlnBP-Gln复合物的溶液结构是必要的。 我们一直
研究GlnBP的骨架NMR共振归属,
分配了大约95%的残基。 现在我们开始研究
侧链分配和结构确定。 H(CCO)NH和4D
NOESY实验预计将为此目的运行。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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CHARLES R HUTCHINSON其他文献
CHARLES R HUTCHINSON的其他文献
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