CRYSTAL STRUCTURE OF MTCP 1, AN ONCOGENE EXPRESSED IN T CELL MALIGNANCIES

成熟T细胞增殖因子1（MTCP-1）和TCL-1基因形成 一个新的家族，涉及T细胞恶性肿瘤和淋巴 增殖 TCL-1和MTCP-1癌基因编码14kDa蛋白 共享41%相同的氨基酸残基，但没有显著差异， 与其他已知的蛋白质相似。 的晶体结构 MTCP-1将用于设计突变以研究功能， 作为治疗T细胞的潜在药物的肽类似物 恶性肿瘤。 MTCP-1晶体在空间群P6中生长，单位为 晶胞尺寸a = B = 61.41和c = 87.31
. 晶体 可调节至2.5 - 2.0
分辨率，三位重原子 衍生物已被发现，和阶段的结果，部分 可解释电子密度图。 有两个问题： X射线数据很弱，辐射衰减降低了测量的准确性。 测量;并且衍生物不是相互同构的。 同步辐射时间要求获得准确的高分辨率数据 对于衍生品的原生和异常数据， 改善阶段。 我们还准备了富含硒代蛋氨酸的 用于MAD定相的MTCP-1。

Mature T-cell Proliferation Factor 1 (MTCP-1) and TCL-1 genes form a novel family that is involved in T-cell malignancies and lymphoid proliferation. TCL-1 and MTCP-1 oncogenes encode for 14kDa proteins that share 41% identical amino acid residues but have no significant similarities with other known proteins. The crystal structure of MTCP-1 will be used to design mutations to study the function, and peptide analogs as potential drugs for treatment of T-cell malignancies. Crystals of MTCP-1 grow in space group P6 with the unit cell dimensions of a = b = 61.41 and c = 87.31
. The crystals diffract to 2.5-2.0
resolution, three potential heavy atom derivatives have been found, and phases result in a partially interpretable electron density map. There are two problems: the X-ray data are weak and radiation decay reduces the accuracy of the measurements; and the derivatives are not mutually isomorphous. Synchrotron time is requested to obtain accurate high resolution data for the native and anomalous data for the derivatives in order to improve the phases. We are also preparing selenomethionine enriched MTCP-1 for MAD phasing.