CRYSTAL STRUCTURE OF MTCP 1, AN ONCOGENE EXPRESSED IN T CELL MALIGNANCIES

成熟T细胞增殖因子1和TCL-1基因形成 一个与T细胞恶性肿瘤和淋巴组织相关的新家族 扩散。TCL-1和MTCP-1癌基因编码14 kDa蛋白 它们有41%相同的氨基酸残基，但没有显著的 与其他已知蛋白质的相似性。该化合物的晶体结构 MTCP-1将用于设计突变以研究其功能，以及 多肽类似物作为治疗T细胞的潜在药物 恶性肿瘤。用该装置在P6空间群生长MTCP-1晶体 晶胞尺寸a=b=61.41和c=87.31。水晶 衍射率为2.5-2.0分辨率，三势重原子 已经发现了导数，并且相导致了部分 可解释的电子密度图。有两个问题： X射线数据很弱，辐射衰变降低了 测量；并且这些导数不是相互同构的。 需要同步加速器时间才能获得准确的高分辨率数据 对于衍生工具的本机数据和异常数据，以便 完善各阶段。我们还在准备富硒蛋氨酸 用于疯狂相移的MTCP-1。

Mature T-cell Proliferation Factor 1 (MTCP-1) and TCL-1 genes form a novel family that is involved in T-cell malignancies and lymphoid proliferation. TCL-1 and MTCP-1 oncogenes encode for 14kDa proteins that share 41% identical amino acid residues but have no significant similarities with other known proteins. The crystal structure of MTCP-1 will be used to design mutations to study the function, and peptide analogs as potential drugs for treatment of T-cell malignancies. Crystals of MTCP-1 grow in space group P6 with the unit cell dimensions of a = b = 61.41 and c = 87.31
. The crystals diffract to 2.5-2.0
resolution, three potential heavy atom derivatives have been found, and phases result in a partially interpretable electron density map. There are two problems: the X-ray data are weak and radiation decay reduces the accuracy of the measurements; and the derivatives are not mutually isomorphous. Synchrotron time is requested to obtain accurate high resolution data for the native and anomalous data for the derivatives in order to improve the phases. We are also preparing selenomethionine enriched MTCP-1 for MAD phasing.