STEM MASS MAPPING & HEAVY ATOM LABELING OF BIOMOLECULES

布鲁克海文STEM（扫描透射电子显微镜）资源 提供了一个独特的服务质量和重原子簇映射的 单个生物分子。 STEM I将继续定位重型 原子簇和映射投影质量分布，得到1-2 nM 有用的空间分辨率和2-10%的质量分辨率， 病毒在低剂量下成像。 STEM III已接近完成， 提高分辨率、样本处理和数据处理能力 收藏. 我们建议继续扩大我们的高效用户/合作者计划 如位点特异性标记、标本制备、成像和成像 分析方法的发展。 目前，有65个项目在这些地区开展活动， 蛋白质和蛋白质复合物，细丝和丝状蛋白质， 膜和膜蛋白、DNA、RNA和核酸/蛋白质 复合物，病毒蛋白质和病毒，重原子簇和冷冻 电镜预计所有项目将继续导致 在最高质量的期刊上发表文章。 核心研发计划的主要重点将继续是量化 了解样品制备以及改进的 重原子簇标记，改进的显微镜，改进的成像 技术（包括使用特定能量的低剂量元素映射 损失）和改进的图像分析方法。

The Brookhaven STEM (Scanning Transmission Electron Microscopy) Resource provides a unique service for mass and heavy atom cluster mapping of individual biological molecules. STEM I will continue localizing heavy atom clusters and mapping projected mass distribution, giving 1-2 nM useful spatial resolution and 2-10% mass resolution for objects as large as viruses imaged at low dose. STEM III is nearing completion with improved capabilities in resolution, specimen handling and data collection. We propose to continue to expand our productive user/collaborator program as site-specific labeling, specimen preparation, imaging and image analysis methods develop. Currently 65 projects are active in the areas of: protein and protein complexes, filaments and filamentous proteins, membranes and membrane proteins, DNA, RNA and nucleic acid/protein complexes, viral proteins and viruses, heavy atom cluster and cryo electron microscopy. All projects are expected to continue to lead to publications in the highest quality journals. Major emphasis of the core R&D program will continue to be quantitative understanding of specimen preparation as well as development of improved heavy atom cluster labels, improved microscopes, improved imaging techniques (including low dose elemental mapping using specific energy losses) and improved image analysis methods.