TRAINING ACTIVITY AT THE CENTER

中心的培训活动

• 批准号: 6120595
• 负责人: JOSEPH S WALL
• 金额: $ 2.14万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120595
• 关键词: animal tissue; biomedical resource; genome; growth /development

A study of the shucture and function of a microtubule-nucleating complex from Drosophild embryo extracts was initiated. The y-TuRC (y-Tubulin Ring Complex) appeared as disc-shaped particles in the STEM when the specimen was fi=ze-dried, simila to previous TEM image& The preparation was relatively clean and the complexes were approximately 2.5 MDa. Many additional experiments are planned. It turns out that their preparations routinely have 2kDa peptides (to elute the complex from a column) which has'caused bad background problems. Work is underway to avoid this.

一种微管成核器的结构与功能研究 从果蝇胚胎提取物的复合物开始。 Y-TuRC （γ-微管蛋白环复合物）在STEM中表现为盘状颗粒 当样品被过滤干燥时，类似于先前的TEM图像， 制备相对清洁，复合物约为 2.5 MDa。 许多额外的实验正在计划中。 事实证明 他们的制剂通常含有2kDa的肽（以使复合物稳定 从一个专栏），这已经造成了不良的背景问题。 工作是 正在进行中，以避免这种情况。