CORE--PROTEIN CHEMISTRY

核心--蛋白质化学

• 批准号: 6235781
• 负责人: CLIVE A SLAUGHTER
• 金额: $ 5.04万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-10 至 1998-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6235781
• 关键词: biomedical facility; cellular pathology; computer assisted sequence analysis; electrofocusing; enzyme linked immunosorbent assay; gel electrophoresis; gel filtration chromatography; glycoproteins; high performance liquid chromatography; immunoelectrophoresis; ion exchange chromatography; laboratory rabbit; mass spectrometry; peptide chemical synthesis; protein purification; protein sequence; skin disorder; western blottings

The mission of the Protein Chemistry Core is to provide the facilities, services, and technical expertise necessary for purification, identification and chemical characterization of proteins that are important to the success of the research efforts of the participating investigators. The specific aims are (1) to advise SDRC investigators on identifying, purifying and characterizing proteins and glycoproteins of interest that play critical roles in cell-cell and cell-extracellular matrix interactions within the skin, and (2) to provide services for purification, accurate amine acid sequencing, determination of amino acid composition, mass, and homology relationships of proteins of interest, and for the production of synthetic peptides for functional studies and antiserum generation.

蛋白质化学核心的使命是提供 必要的设施、服务和技术专长 纯化、鉴定和化学表征 对研究工作的成功很重要的蛋白质 参与的调查人员。 具体目标是 (1) 提供建议 SDRC 研究人员进行鉴定、纯化和表征 感兴趣的蛋白质和糖蛋白在 皮肤内细胞与细胞和细胞与细胞外基质的相互作用， (2)提供纯化、精确氨基酸的服务 测序、氨基酸组成、质量的测定，以及 感兴趣的蛋白质的同源关系，以及 生产用于功能研究的合成肽和 抗血清的产生。