Beckman XLI Analtical Ultracentrifuge

描述（由申请人提供）：分析性超离心是测定溶液中大分子复合物质量和形状的首选方法。经过30多年的文献研究，该方法得到了很好的验证。近年来，仪器和数据分析软件的进步使这种方法更容易使用。分子相互作用的研究在过去几年中获得了新的流行，作为基因组和蛋白质组学研究的合乎逻辑的下一步。Hartwell中心是St. Jude儿童研究医院（SJCRH）的生物技术和生物信息学核心设施，正在寻求扩大其分子相互作用能力，以满足医院研究项目的要求。了解多蛋白质和蛋白质- dna复合物的组成和结构是这项工作的主要部分。我们已经确定了SJCRH正在进行的五个迫切需要这项技术的主要研究项目。这些研究项目涵盖了广泛的研究领域，包括免疫学、传染病学、药理学、药效学和结构生物学。还有许多其他的项目也有类似的需求，或者在不久的将来会有类似的需求。目前，这项技术还没有在SJCRH或孟菲斯市使用。本申请寻求购买一台分析性超离心机的资金，以支持这些nih支持的研究工作。该仪器将并入圣裘德儿童研究医院的哈特韦尔生物信息学和生物技术中心，以满足当前和未来基础生物医学研究的需要。

DESCRIPTION (provided by applicant): Analytical ultracentrifugation is the method of choice for determination of mass and shape of macromolecular complexes in solution. With over 30 years of literature on the technique, the method is well established. In recent years, advances in the instrumentation and data analysis software have made the method generally more accessible. The study of molecular interactions has gained a new popularity in the past few years as a logical next step to genomic and proteomic studies. The Hartwell Center, a biotechnology and bioinformatics core facility at St. Jude Children's Research Hospital (SJCRH), is seeking to expand its molecular interactions capabilities to meet the requirements of the Hospital's research programs. Understanding the composition and structure of multi-protein and protein-DNA complexes is a major part of this effort. We have identified five major research programs underway at SJCRH with immediate need for this technology. These research programs cover a broad range of studies including Immunology, Infectious Disease, Pharmacology, Pharmacodynamics, and Structural Biology. There are many other programs that have similar needs or will have in the near future. Currently, this technology is not available at SJCRH or in the city of Memphis. This application seeks funding for the purchase of an analytical ultracentrifuge to support these NIH-supported research efforts. The instrument will be incorporated into the Hartwell Center for Bioinformatics and Biotechnology at St. Jude Children's Research Hospital for present and future needs in basic biomedical research.