SOUTHERN CALIFORNIA AIDS MALIGNANCY NETWORK

加州大学洛杉矶分校和南加州大学艾滋病项目将标本库作为资源 为各自的研究社区提供实验室。因为 这些知识库中的每一个都有独特的资源， 合作存在。这些实验室旨在支持临床 试验以及基础科学和流行病学调查， 艾滋病相关恶性肿瘤的发病机制。Cedars Sinai医疗中心 作为转诊中心的社区三级护理医院 在洛杉矶进行艾滋病相关淋巴瘤病理学研究。综合这些 三个中心获得了200多例新的卡波西肉瘤病例和100例 淋巴瘤每年 在加州大学洛杉矶分校，实验室拥有超过15000个计算机化库存， 血清样本、600份血浆样本和800份冷冻活淋巴细胞 准备工作该银行提供分离的卡波西肉瘤细胞和艾滋病 用于基础科学研究的淋巴瘤细胞。样本链接到 临床状态和结果作为参与临床 审判大多数患者有超过5年的多个样本， 纵向数据在南加州大学，实验室拥有超过5000份血清样本， 306份尿样，84份卡波西肉瘤病变的RNA/组织制备物， 以及超过20种新鲜的卡波西肉瘤细胞系。大多数样本 从一项广泛的流行病学调查中收集， 阐明导致卡波西病发展的病原体或过程 肉瘤或来自抗KS和抗淋巴瘤剂的临床试验。 合作者使用这些样本排除Rochelimea作为致病因素 KS试剂，检查细胞因子扰动和 淋巴瘤的发展，确定后受体中的关键分子 KS中导致增殖的信号转导途径，证明 淋巴瘤中重链的利用是非随机的， 艾滋病恶性肿瘤的克隆性，和分离的基因片段， 可能与经典和艾滋病相关KS有关的生物体。在雪松 西奈半岛，超过235例艾滋病相关淋巴瘤已被分析， 约会这些研究包括广泛的免疫表型表征， EBV研究和p53突变的描述。到目前为止，这三个 资料库为多个机构的25名以上调查人员提供了服务 对需要临床材料的广泛的基础科学问题进行研究。 这些银行的独特能力，提供罕见的标本（如女性 或HIV阴性流行性KS细胞系）和相关的临床 将结果置于背景中所需的信息是最大的 这个仓库的力量。 本申请是一个联合储存库，以存放与以下方面有关的样本： 艾滋病相关的恶性肿瘤在加州大学洛杉矶分校后，在当地的网站处理。的 应用程序混合每个样品的单独强度， 拥有艾滋病恶性肿瘤专家的知识库 investigators. 该银行已经拥有了大量的样本， Kaposi肉瘤与淋巴瘤发病机制研究有价控制 来自匹配的社区对照的样本是唯一的 仓库。 合并后的存储库将继续收集控制权 样本、临床试验患者样本、流行病学数据，以及 临床结果数据。 储藏室将提供血清， 来自AIDS恶性肿瘤患者的血浆和尿液。 总RNA 制备、新鲜和石蜡包埋的淋巴瘤和KS组织样本 将沿着提供基线流行病学数据和临床 反应

The UCLA and USC AIDS programs operate specimen repositories as resource laboratories for their respective research communities. Because of the unique resources of each of these repositories, significant extramural collaborations exist. These laboratories are designed to support clinical trials as well as basic science and epidemiologic investigations of the pathogenesis of AIDS related malignancies. Cedars Sinai Medical Center is a community based tertiary care hospital which acts as the referral center for AIDS related lymphoma pathology studies in Los Angeles. Together these three centers access over 200 new cases of Kaposi sarcoma and 100 cases of lymphoma each year. At UCLA, the laboratory' houses a computerized inventory of over 15,000 serum samples, 600 plasma samples and 800 frozen viable lymphocyte preparations. The bank provides isolates of Kaposi sarcoma cells and AIDS lymphoma cells for basic science studies. The samples are linked to clinical status and outcome as a result of participation in clinical trials. Most patients have multiple samples dating over 5 years providing longitudinal data. At USC, the laboratory houses over 5000 serum samples, 306 urine samples, 84 RNA/tissue preparations of Kaposi sarcoma lesions, and over 20 fresh Kaposi sarcoma cell lines. The majority of the samples were collected from an extensive epidemiologic survey designed to elucidate pathogens or processes leading to the development of Kaposi sarcoma or from clinical trials of anti-KS and anti-lymphoma agents. Collaborators have used these samples to exclude Rochelimea as a causative agent for KS, examine the relationship between cytokine perturbations and the development of lymphomas, identify key molecules in the post-receptor pathway of signal transduction in KS leading to proliferation, demonstrate that heavy chain utilization in lymphomas is non-random, investigate the clonality of AIDS malignancies, and isolated genetic fragments of a organism that maybe linked to classical and AIDS-related KS. At Cedars Sinai, over 235 cases of AIDS related lymphomas have been analyzed to date. These studies include extensive immunophenotypic characterization, EBV studies and delineation of p53 mutations. To date, these three repositories have serviced over 25 investigators at multiple institutions on a wide range of basic science questions requiring clinical materials. The unique ability of these banks to provide rare specimens (such a female or HIV negative epidemic KS cell lines) and the associated clinical information needed to place the results in context are the greatest strength of this repository. This application is for a joint repository to house samples related to AIDS related malignancies at UCLA after processing at the local sites. The application blends the individual strengths of each of the sample repositories with the talents of accomplished AIDS malignancies investigators. The bank already contains a wealth of samples vital to the study of Kaposi sarcoma and lymphoma pathogenesis. Valuable control samples from matched community based controls are unique to this repository. The combined repository will continue to collect control samples, samples from patients on clinical trials, epidemiologic data, and clinical outcome data. The repository will provide access to serum, plasma, and urine from patients with AIDS malignancies. Total RNA preparations, fresh and paraffin embedded lymphoma and KS tissue samples will be available along with the baseline epidemiologic data and clinical response.