CORE--ESTABLISHMENT OF CELL LINES AND TUMORIGENICITY TESTING

核心--细胞系建立及致瘤性检测

• 批准号: 6236712
• 负责人: BEPPINO C GIOVANELLA
• 金额: $ 17.91万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6236712
• 关键词: Wilms' tumor; athymic mouse; biomedical facility; cancer registry /resource; carcinoma; human tissue; neoplasm /cancer transplantation; neoplastic cell; osteosarcoma; sarcoma; tissue /cell culture; tissue resource /registry; transplantation resource /registry /referral center; xenotransplantation

The essential task of our group is to provide material for use by the other groups. Two types of material are needed: tumor tissue from the patients when available (Wilm's tumors but also other malignancies appearing in the families under study, osteosarcomas, soft tissue sarcomas, breast carcinomas, etc.), and normal cells from all the members of said families. In order to have a continuous source of tumor material, fragments of Wilm's tumors or of other malignancies, obtained when surgery is performed on the patient, are transplanted subcutaneously in nude mice. If a positive take is obtained, the tumor is serially passaged in other nudes, expanded, viably frozen for further reference and supplied in any quantity requested to the other groups of our program. In order to have a continuous source of normal cells from which DNA, RNA and proteins can be extracted, blood samples obtained from patients and relatives are processed, their lymphocytes transformed by EBV and lymphoblastoid cell lines (LCL) obtained, propagated, expanded, viably frozen and supplied in any quantity requested to the other groups of our program. Fibroblasts are cultured from normal tissue biopsies obtained also from patients and relatives when feasible.

我们小组的基本任务是提供材料供 其他各组需要两种类型的材料： 患者（Wilm肿瘤以及其他恶性肿瘤） 出现在研究的家族中，骨肉瘤，软组织 肉瘤、乳腺癌等），和正常细胞 说的家庭。 为了获得肿瘤物质的连续来源， Wilm's肿瘤或其他恶性肿瘤，手术时获得 在患者身上，被皮下移植到裸鼠中。 如果 获得阳性，将肿瘤在其他裸鼠中连续传代， 膨胀，可冷冻以供进一步参考，并以任何数量供应 向我们节目的其他组提出了要求。 为了有一个正常细胞的连续来源， 可以提取蛋白质，从患者获得的血液样品， 亲属被处理，他们的淋巴细胞被EBV转化， 获得、增殖、扩增、存活的淋巴母细胞样细胞系（LCL） 冷冻并按要求的任何数量供应给我们的其他团体 程序. 成纤维细胞是从正常组织活检中培养的， 患者和家属也可以。