CORE--MOLECULAR ANALYSIS OF P53 TUMOR SUPPRESSOR GENE

核心--P53抑癌基因的分子分析

• 批准号: 6236715
• 负责人: MARC F HANSEN
• 金额: $ 17.91万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6236715
• 关键词: biomedical facility; denaturing gradient gel electrophoresis; family genetics; gene mutation; genetic markers; genetic polymorphism; germ cells; human genetic material tag; method development; multiple primary neoplasia; neoplasm /cancer genetics; nucleic acid sequence; pediatric neoplasm /cancer; polymerase chain reaction; restriction fragment length polymorphism; sarcoma; southern blotting; tumor suppressor genes

One of the critical requirements for the success of this program will be the ability to identify heterozygous p53 mutations in the constitutional tissues of probands and their families. The foundation of this program is the examination of relatively large numbers of samples for what could be many different single base mutations. The success of this project requires the accurate and rapid identification of mutations in the p53 gene from either constitutional or tumor tissues. This core has been proposed to develop the techniques necessary to identify these mutations, and then to examine the probands or nearest affected relatives from the cohorts to determine the frequency of p53 mutations in these families and individuals.

该计划成功的关键要求之一是 鉴定体质性肿瘤中杂合p53突变的能力， 先证者及其家属的组织。该计划的基础是 对相对大量的样本进行检查， 许多不同的单碱基突变。这个项目的成功需要 p53基因突变的准确和快速鉴定， 无论是体质组织还是肿瘤组织。该核心已被提议用于 开发必要的技术来识别这些突变，然后 检查队列中的先证者或最近的受影响亲属， 确定这些家族中p53突变的频率， 个体