CORE--IMMUNOCYTOCHEMISTRY AND ISLET ISOLATION LABORATORY

核心--免疫细胞化学和胰岛分离实验室

• 批准号: 6239308
• 负责人: MICHELE SOLIMENA
• 金额: $ 16.91万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6239308
• 关键词: biomedical facility; cell line; confocal scanning microscopy; genetically modified animals; histology; immunocytochemistry; laboratory mouse; pancreatic islets; tissue /cell culture

The purpose of the Immunocytochemistry and Islet Isolation Core Facility is to fold: 1) to make available to the team of the Project a variety of immunocytochemical techniques for the analysis of cells and tissues with state of the art technology; 2) to supply the individual investigators with freshly isolated pancreatic islets from ice. The facility will offer technical and scientific expertise for immunocytochemical studies using conventional and confocal light microscopy as well as provide services in cryomicrotomy and immuncytochemistry for the histological analysis of tissues and cell lines from normal and trangenic animals. These techniques will be applied to the study of proteins such as MHC molecules, T cell receptors, cytokines, and beta-cell utoantigens, including GAD, insulin and ICA512. Routine isolation of freshly pancreatic islet cells from NOD and transgenic mice will be required for generating and maintaining islet- specific T cell clones. A centralized facility has the advantage of assuring that islets for the different projects are prepared using standardized and up-to-date procedures.

免疫细胞化学和胰岛分离核心的目的 设施包括：1）向项目团队提供 用于细胞分析的多种免疫细胞化学技术， 具有最新技术的纸巾; 2）为个人提供 研究人员用新鲜分离的胰岛从冰。 的 设施将提供技术和科学知识， 使用常规和共聚焦光的免疫细胞化学研究 显微镜，并提供服务，在冷冻显微镜， 免疫细胞化学用于组织和细胞的组织学分析 来自正常和转基因动物的细胞系。 这些技术将 应用于蛋白质如MHC分子、T细胞 受体、细胞因子和β细胞抗原，包括GAD、胰岛素 ICA512。 常规分离来自NOD的新鲜胰岛细胞， 需要转基因小鼠来产生和维持胰岛- 特异性T细胞克隆。 集中式设施的优点是 确保为不同项目准备胰岛， 标准化和最新的程序。