CORE--IMMUNOCYTOCHEMISTRY AND ISLET ISOLATION LABORATORY

免疫细胞化学和胰岛分离核心的作用 设施是折叠：1)向项目团队提供a) 多种免疫细胞化学技术用于分析细胞和 具有最先进技术的纸巾；2)为个人提供 研究人员刚刚从冰中分离出胰岛。这个 设施将为以下方面提供技术和科学专业知识 常规光和共聚焦光免疫细胞化学研究 以及提供冷冻显微切割术和 免疫细胞化学用于组织和细胞的组织学分析 来自正常动物和转基因动物的线条。这些技术将是 应用于MHC分子、T细胞等蛋白质的研究 受体、细胞因子和β细胞自身抗原，包括GAD、胰岛素 和ICA512。 新鲜胰岛细胞的常规分离 将需要转基因小鼠来产生和维持胰岛- 特定的T细胞克隆。集中式设施的优势在于 确保为不同项目准备的小岛是使用 标准化和最新的程序。

The purpose of the Immunocytochemistry and Islet Isolation Core Facility is to fold: 1) to make available to the team of the Project a variety of immunocytochemical techniques for the analysis of cells and tissues with state of the art technology; 2) to supply the individual investigators with freshly isolated pancreatic islets from ice. The facility will offer technical and scientific expertise for immunocytochemical studies using conventional and confocal light microscopy as well as provide services in cryomicrotomy and immuncytochemistry for the histological analysis of tissues and cell lines from normal and trangenic animals. These techniques will be applied to the study of proteins such as MHC molecules, T cell receptors, cytokines, and beta-cell utoantigens, including GAD, insulin and ICA512. Routine isolation of freshly pancreatic islet cells from NOD and transgenic mice will be required for generating and maintaining islet- specific T cell clones. A centralized facility has the advantage of assuring that islets for the different projects are prepared using standardized and up-to-date procedures.