CORE--PERIPHERAL STEM CELL LABORATORY

核心--外周干细胞实验室

• 批准号: 6239214
• 负责人: Leonard Stanley Sender
• 金额: $ 13万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-10 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6239214
• 关键词: biomedical facility; blood treatment; flow cytometry; hematopoietic stem cells; human subject; tissue /cell culture; tissue resource /registry; transfection

The primary function of the Peripheral Stem Cell Core Laboratory will be to act as the conduit for the processing of reconstituting hematopoietic stem cells, isolated, purified and/or expanded from either human bone marrow, peripheral or placental blood. This central core laboratory will ensure the quality control of all stem cell materials distributed to other laboratories for research purposes, adherence to all current and future regulatory requirements and cost control for all projects, by bulk central purchasing agreements and by the efficient and integrated utilization of supplies. The Peripheral Stem Cell Core Laboratory (SCL) will have two interactive compounds, i) the Apheresis Unit to collect peripheral blood stem cells (PBSC) from normal donors and patients and ii) the Peripheral Stem Cell Research Laboratory. Apheresis services include the collection of peripheral blood stem cells from patients, the collection of allogeneic single donor platelets and peripheral blood stem cells for research purposes from normal volunteer donors. Services to be provided in the Peripheral Stem Cell Research Laboratory, include: i) Isolation, purification and ex-vivo expansion of stem cell populations from marrow, peripheral and placental blood, ii) Analysis of stem cell populations by flow cytometry and immunohistochemistry, iii) Cryopreservation and storage of stem cell populations for research use, iv) Disbursement of purified, expanded and/or cryopreserved stem cell populations for research purposes and v) Development and implementation of clinical protocols for gene transfer into hematopoietic stem cell populations.

外周干细胞核心实验室的主要功能是