Development and application of eDNA tools to assess the structure and function of coastal sea ecosystems (MARINe-DNA)

评估沿海海洋生态系统结构和功能的eDNA工具的开发和应用（MARINe-DNA）

• 批准号: NE/N006151/1
• 负责人: Michael Cunliffe
• 金额: $ 30.12万
• 依托单位: Marine Biological Association of the United Kingdom
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FN006151%2F1
• 关键词: Development; application; eDNA; tools; assess

This NERC highlight topic focuses on the use of eDNA as a new tool for 21st century ecology. Environmental DNA (eDNA) is defined in the call as 'free DNA present outside of any organism'. The aim of the call is to address the current knowledge gaps in the application of eDNA approaches to help understand community biodiversity and dynamics of ecosystem functioning. We will conduct a proof-of-concept investigation at Station L4, an exemplar coastal ocean ecosystem, and natural laboratory, in the English Channel off Plymouth, UK. Starting with a hydrodynamic model to spatially and temporally define the ecosystem (how large is the natural laboratory?) the project will then be split into three experimental phases: 1) eDNA methodological validation (developing the tools);2) 18-month temporal pelagic survey (testing the tools); and3) Comprehensive data analysis and model assimilation (did the tools work, what did they tell us, and are they useful?)Using a wide range of expertise from 4 different institutions (PML, MBA, NOC, and U.Exeter), we will investigate a spatially defined region, from estuarine to coastal, benthic to pelagic; and at a range of temporal resolutions building on NERC National Capability sampling regimes and biosensor deployment. E-metagenetic and e-metagenomic data (individual genes to whole genomes) will be used to answer cross-cutting science questions utilising current physicochemical and biological information collected in parallel at this important coastal site. Results from this project will provide a methodological template for the use of eDNA and remote eDNA biosensors in aquatic ecosystems. Downstream data will significantly advance our understanding of persistence of eDNA, and its potential impact on informing models of ecosystem functioning. Products of this research will have wider implications for the use of this tool on fisheries assessments, fish pathogen detection, conservation biology, environmental risk management (e.g. toxic algae blooms, human pathogens, ballast water regulations), with the wider aim of supporting biodiversity and nature's services through NERC's strategic pillar of "Managing environmental change".

这个NERC重点主题重点是将Edna用作21世纪生态的新工具。环境DNA（EDNA）在呼叫中定义为“在任何有机体之外存在的自由DNA”。呼吁的目的是解决EDNA方法应用中当前的知识差距，以帮助了解社区生物多样性和生态系统功能的动态。我们将在英国普利茅斯附近的英式海峡沿海地区沿海地区生态系统和自然实验室进行概念验证调查。从水平动力学模型开始，以在空间和时间上定义生态系统（自然实验室有多大？）然后将项目分为三个实验阶段：1）Edna方法论验证（开发工具）; 2）18个月的暂时性上层调查（测试工具）； 3）使用来自四个不同机构（PML，MBA，MBA，NOC和U.Eset）的广泛专业知识的全面数据分析和模型同化（工具有效，它们是否有用？以及在NERC国家能力采样制度和生物传感器部署的一系列时间决议中。电子基因因和电子元素数据（整个基因组的个别基因）将用于回答跨裁切科学问题，利用当前在这个重要沿海地点并行收集的当前物理化学和生物学信息。该项目的结果将为水生生态系统中使用EDNA和远程EDNA生物传感器提供方法学模板。下游数据将大大提高我们对EDNA持久性的理解，及其对了解生态系统功能模型的潜在影响。这项研究的产品将对该工具在渔业评估，发现，保护生物学，环境风险管理（例如有毒藻类盛开，人类病原体，压肢水法规）上的使用具有更大的影响，并通过NERC通过NERC的策略环境改变来支持生物多样性和大自然的服务。

项目成果

Distinct Oceanic Microbiomes From Viruses to Protists Located Near the Antarctic Circumpolar Current.

• DOI: 10.3389/fmicb.2018.01474
• 发表时间: 2018
• 期刊: Frontiers in microbiology
• 影响因子: 5.2
• 作者: Flaviani F;Schroeder DC;Lebret K;Balestreri C;Highfield AC;Schroeder JL;Thorpe SE;Moore K;Pasckiewicz K;Pfaff MC;Rybicki EP
• 通讯作者: Rybicki EP

A 17-year time-series of fungal environmental DNA from a coastal marine ecosystem reveals long-term seasonal-scale and inter-annual diversity patterns.

• DOI: 10.1098/rspb.2022.2129
• 发表时间: 2023-02-08
• 期刊: PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
• 影响因子: 4.7
• 作者: Chrismas, Nathan;Allen, Ro;Allen, Michael J.;Bird, Kimberley;Cunliffe, Michael
• 通讯作者: Cunliffe, Michael

Complex photobiont diversity in the marine lichen Lichina pygmaea

• DOI: 10.1017/s002531542100062x
• 发表时间: 2021-06-01
• 期刊: JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM
• 影响因子: 1.2
• 作者: Chrismas, Nathan A. M.;Allen, Ro;Cunliffe, Michael
• 通讯作者: Cunliffe, Michael

Limited congruence exhibited across microbial, meiofaunal and macrofaunal benthic assemblages in a heterogeneous coastal environment.

• DOI: 10.1038/s41598-018-33799-9
• 发表时间: 2018-10-19
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Cronin-O'Reilly S;Taylor JD;Jermyn I;Allcock AL;Cunliffe M;Johnson MP
• 通讯作者: Johnson MP

Multi-year assessment of coastal planktonic fungi reveals environmental drivers of diversity and abundance

• DOI: 10.1038/ismej.2016.24
• 发表时间: 2016-09-01
• 期刊: ISME JOURNAL
• 影响因子: 11
• 作者: Taylor, Joe D.;Cunliffe, Michael
• 通讯作者: Cunliffe, Michael