DOPAMINE FUNCTION IN MIDBRAIN
DOPAMINE FUNCTION IN MIDBRAIN
批准号:
6233841
负责人:
LOUIS A CHIODO
金额:
$18.78万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1996
资助国家:
美国
项目状态:
已结题
起止时间:
1996-12-01 至 1998-11-30
关键词:
G protein RNase protection assay action potentials biological signal transduction developmental neurobiology dopamine dopamine receptor dopamine transporter electrophysiology embryo /fetus tissue /cell culture embryo /fetus toxicology enkephalins flow cytometry gene expression high performance liquid chromatography immunocytochemistry in situ hybridization laboratory rat membrane transport proteins mesencephalon neuropharmacology neurotransmitter biosynthesis neurotransmitter transport newborn animals northern blottings pregnancy receptor binding receptor sensitivity substance P teratogens tyrosine 3 monooxygenase
中文摘要
在子宫内接触乙醇的动物和人类表现出运动改变
活动和增加的觉醒。 基于广泛的临床和
临床前文献表明这些功能是
受中脑多巴胺(DA)系统的影响,我们假设
这些神经元系统可能会因产前乙醇暴露而改变。
该提案中详细说明的初步观察结果表明,
假设是正确的。 该提案详细介绍了对
子宫内乙醇暴露对多巴胺神经系统的影响。
它概述了一系列相互关联的研究,这些研究将检验变化
在突触前多巴胺神经元和突触后多巴胺敏感神经元中
细胞。 分析将包括详细调查
多巴胺神经元的数量和形态,多巴胺细胞的评估
电生理学(放电频率和模式),检查变化
多种多巴胺受体(多巴胺自身受体、D2 和 D1
受体),检查改变的 G 蛋白 mRNA 表达,分析
P 物质和甲啡肽 mRNA 表达的检测,以及
多巴胺转运蛋白。 结合电生理学,
免疫组织化学、生化和药理学方法
受雇。 希望所获得的发现能够提供新的
有关乙醇对表达的影响的信息
中脑多巴胺神经系统的正常成人特征。
英文摘要
Animals and humans exposed to ethanol in utero exhibit altered motor
activity and increased arousal. Based on an extensive clinical and
preclinical literature which indicates that these functions are
influenced by mesencephalic dopamine (DA) systems, we hypothesized that
these neuronal systems might be altered by prenatal ethanol exposure.
Preliminary observations detailed within this proposal indicate that this
hypothesis was correct. This proposal details a complete examination of
the influence of in utero ethanol exposure on dopamine neuronal systems.
It outlines a series of interrelated studies which will examine changes
in both presynaptic dopamine neurons and postsynaptic dopamine-sensitive
cells. The analyses will include the detailed investigation of the
number and morphology of dopamine neurons, evaluation of dopamine cell
electrophysiology (firing rate and pattern), examination of the changes
in various dopamine receptors (dopamine autoreceptors, D2 and D1
receptors), examination of altered G-protein mRNA expression, analysis
of substance P and met-enkephalin mRNA expression, and examination of the
dopamine transporter. Combined electrophysiological,
immunohistochemical, biochemical and pharmacological methods will be
employed. It is hoped that the findings obtained will provide new
information regarding the influence of ethanol on the expression of
normal adult characteristics of mesencephalic dopamine neuronal systems.
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DOPAMINE FUNCTION IN MIDBRAIN
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批准号:6097669
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项目类别:
-
资助金额:$0.0万
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财政年份:1998
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负责人:LOUIS A CHIODO
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依托单位:
DOPAMINE FUNCTION IN MIDBRAIN
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批准号:5204246
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:LOUIS A CHIODO
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依托单位:--
PSYCHOPHARMACOLOGY OF DA AGONIST CNS EFFECTS: ACUTE AND CHRONIC STUDIES
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批准号:3891471
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:LOUIS A CHIODO
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依托单位:
海外基金