DOPAMINE FUNCTION IN MIDBRAIN

中脑的多巴胺功能

• 批准号: 6097669
• 负责人: LOUIS A CHIODO
• 金额: --
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6097669
• 关键词: G protein; RNase protection assay; action potentials; biological signal transduction; developmental neurobiology; dopamine; dopamine receptor; dopamine transporter; electrophysiology; embryo /fetus tissue /cell culture; embryo /fetus toxicology; enkephalins; flow cytometry; gene expression; high performance liquid chromatography; immunocytochemistry; in situ hybridization; laboratory rat; membrane transport proteins; mesencephalon; neuropharmacology; neurotransmitter biosynthesis; neurotransmitter transport; newborn animals; northern blottings; pregnancy; receptor binding; receptor sensitivity; substance P; teratogens; tyrosine 3 monooxygenase

Animals and humans exposed to ethanol in utero exhibit altered motor activity and increased arousal. Based on an extensive clinical and preclinical literature which indicates that these functions are influenced by mesencephalic dopamine (DA) systems, we hypothesized that these neuronal systems might be altered by prenatal ethanol exposure. Preliminary observations detailed within this proposal indicate that this hypothesis was correct. This proposal details a complete examination of the influence of in utero ethanol exposure on dopamine neuronal systems. It outlines a series of interrelated studies which will examine changes in both presynaptic dopamine neurons and postsynaptic dopamine-sensitive cells. The analyses will include the detailed investigation of the number and morphology of dopamine neurons, evaluation of dopamine cell electrophysiology (firing rate and pattern), examination of the changes in various dopamine receptors (dopamine autoreceptors, D2 and D1 receptors), examination of altered G-protein mRNA expression, analysis of substance P and met-enkephalin mRNA expression, and examination of the dopamine transporter. Combined electrophysiological, immunohistochemical, biochemical and pharmacological methods will be employed. It is hoped that the findings obtained will provide new information regarding the influence of ethanol on the expression of normal adult characteristics of mesencephalic dopamine neuronal systems.

在子宫内接触乙醇的动物和人类表现出运动改变