INTEGRATED MICROINJECTOR AND IMAGE PROCESSING SYSTEM

这项申请要求为一个综合系统提供资金， 包含以下组件：显微操作- 显微注射装置和带相位的倒置显微镜 对比度、落射荧光和Namarski DIC光学器件以及CCD照相机 图像处理系统。 目前，既没有一个 福克斯大通癌症（FCCC）的可比能力系统，也不是 为这种仪器提供资金。 研究 11名NIH资助的研究人员的利益，他们将是主要的 所要求的仪器的用户差别很大。 但他们的 所有的研究工作都集中在对培养的 微观层面的细胞。 购置所要求的系统 将允许每个研究者的研究从分析扩展到 将细胞群的数量提升到单个细胞的水平。 的各种 所要求的系统的组成部分是根据多样性选择的 这项研究的兴趣，以满足目标， 调查人员既考虑到当前的需要，也考虑到长期的需要。 本文所述的所有研究项目的最终目标 应用是为了了解人类疾病的过程。 这将是 通过研究细胞形态的调节来完成， 肿瘤抑制因子，癌基因，信号转导 分子、病毒和细胞极性修饰剂。 许多 在这些研究中将要研究的细胞效应物有 已经通过分析各种基因的突变来确定， 人类的恶性状态。 一些提议的实验 描述丁型肝炎和B型肝炎病毒分析 复制，这可能最终有助于治疗肝炎， 一种与人类显著发病率相关的疾病， 是肝细胞癌的主要病因。 别人 涉及分析与癌症有关的逆转录病毒系统， 艾滋病 所有这些项目都有一个共同的需求， 光学、显微操作和显微图像处理 能力，这一要求可以最有效地满足 访问请求的系统。 所要求的系统将在 支持在细胞培养设施a气候变化框架公约。 共享设施 是集成系统的理想位置，因为它服务于 大多数NIH资助的实验室在FCCC，是一个枢纽， 与优秀的、训练有素的技术人员合作， 支持.

This application requests funding for an integrated system that incorporates the following components: a micromanipulation- microinjection apparatus, and inverted microscope with phase contrast, epi-fluorescence and Namarski DIC optics and a CCD camera with an image processing system. At present, there is neither a system of comparable capability at Fox Chase Cancer (FCCC) nor is there available funding for such instrumentation. The research interests of the 11 NIH funded investigators who will be the major users of the requested instrumentation vary widely. However, their research efforts all converge in the detailed analysis of cultured cells at the microscopic level. Acquisition of the requested system will permit an extension of each investigator research from analysis of cell populations to the level of single cells. The various components of the requested system were chosen based on the diversity of this research interest, with the goal of satisfying the investigators~ immediate, as well as long term needs. The ultimate goal of all of the research programs described in this application is to understand human disease processes. This will be accomplished through study of the regulation of cell morphology and growth by tumor suppressors, oncogenes, signal transduction molecules, viruses and modifiers of cell polarity. Many of the cellular effectors which will be investigated in these studies have already been identified through analysis of mutations in a variety of malignant states in humans. Some of the proposed experiments describe analysis of hepatitis delta and hepatitis B viral replication, which may ultimately aid in the treatment of hepatitis, a disease that is associated with significant morbidity in humans and represents the leading cause of hepatocellular carcinoma. Others involve analysis of retroviral systems of relevance to cancer and AIDS. All of these projects share a common need for high-resolution optics, micromanipulation and microscope image processing capabilities, a requirement that can be served most effectively by access to the requested system. The requested system will be housed, operated and technically supported in the Cell Culture Facility a FCCC. The shared Facility is an ideal location for the integrated system as it serves the majority of NIH funded laboratories at FCCC and is a hub of collaborative efforts with outstanding, highly trained technical support.