INTEGRATED MICROINJECTOR AND IMAGE PROCESSING SYSTEM

集成显微注射器和图像处理系统

• 批准号: 2593156
• 负责人: ANNA MARIE SKALKA
• 金额: $ 13.8万
• 依托单位: RESEARCH INST OF FOX CHASE CAN CTR
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-15 至 1998-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2593156
• 关键词: biomedical equipment purchase; image processing

This application requests funding for an integrated system that incorporates the following components: a micromanipulation- microinjection apparatus, and inverted microscope with phase contrast, epi-fluorescence and Namarski DIC optics and a CCD camera with an image processing system. At present, there is neither a system of comparable capability at Fox Chase Cancer (FCCC) nor is there available funding for such instrumentation. The research interests of the 11 NIH funded investigators who will be the major users of the requested instrumentation vary widely. However, their research efforts all converge in the detailed analysis of cultured cells at the microscopic level. Acquisition of the requested system will permit an extension of each investigator research from analysis of cell populations to the level of single cells. The various components of the requested system were chosen based on the diversity of this research interest, with the goal of satisfying the investigators~ immediate, as well as long term needs. The ultimate goal of all of the research programs described in this application is to understand human disease processes. This will be accomplished through study of the regulation of cell morphology and growth by tumor suppressors, oncogenes, signal transduction molecules, viruses and modifiers of cell polarity. Many of the cellular effectors which will be investigated in these studies have already been identified through analysis of mutations in a variety of malignant states in humans. Some of the proposed experiments describe analysis of hepatitis delta and hepatitis B viral replication, which may ultimately aid in the treatment of hepatitis, a disease that is associated with significant morbidity in humans and represents the leading cause of hepatocellular carcinoma. Others involve analysis of retroviral systems of relevance to cancer and AIDS. All of these projects share a common need for high-resolution optics, micromanipulation and microscope image processing capabilities, a requirement that can be served most effectively by access to the requested system. The requested system will be housed, operated and technically supported in the Cell Culture Facility a FCCC. The shared Facility is an ideal location for the integrated system as it serves the majority of NIH funded laboratories at FCCC and is a hub of collaborative efforts with outstanding, highly trained technical support.

该申请要求为集成系统提供资金，该系统 包含以下组件：显微操作- 显微注射装置和倒置显微镜 对比、落射荧光和 Namarski DIC 光学器件以及 CCD 相机 具有图像处理系统。 目前，还没有一个 Fox Chase Cancer (FCCC) 的类似能力系统也不是 此类仪器有可用资金。 研究 11 名 NIH 资助的研究人员的利益将成为主要研究人员 所需仪器的用户差异很大。 然而，他们的 研究工作全部集中在对文化的详细分析上 微观水平上的细胞。 获取所请求的系统 将允许每个研究者的研究从分析中延伸出来 细胞群达到单细胞水平。 各种 根据多样性选择所需系统的组件 本研究兴趣的目标是满足 调查人员~即时的以及长期的需求。 本文描述的所有研究计划的最终目标 应用是了解人类疾病过程。 这将是 通过研究细胞形态的调节来完成 肿瘤抑制因子、癌基因、信号转导的生长 分子、病毒和细胞极性修饰剂。 许多 这些研究中将要研究的细胞效应器 通过对多种突变的分析已经确定了 人类的恶性状态。 一些提议的实验 描述丁型肝炎和乙型肝炎病毒的分析 复制，最终可能有助于治疗肝炎， 一种与人类显着发病率相关的疾病 代表肝细胞癌的主要原因。 其他的 涉及与癌症相关的逆转录病毒系统的分析 艾滋病。 所有这些项目都有对高分辨率的共同需求 光学、显微操作和显微镜图像处理 能力，可以最有效地满足这一要求 访问所请求的系统。 所请求的系统将得到安置、操作和技术支持 细胞培养设施 a FCCC 提供支持。 共享设施 是集成系统的理想位置，因为它服务于 大部分 NIH 资助的实验室位于 FCCC，并且是 与优秀、训练有素的技术人员的协作努力 支持。