IRON ASSIMILATION BY NEISSERIA GONORRHOEAE

淋病奈瑟菌在人体内的定植部位有 多样化，每个站点都代表着一个独特的利基市场 营养、环境因素和相互竞争的微生物。这个 生长环境对新陈代谢和细胞生长有显著影响。 淋病奈瑟氏菌的组成，而改变的细胞组成是 通常在这种微生物与人类宿主的相互作用中(1)。 这个项目的总体目标是阐明具体的机制。 被淋病奈瑟氏菌利用来同化 营养铁。父项目的大部分都集中在一项研究上 奈瑟氏菌的主要铁结合蛋白，通常被称为 FBP(铁结合蛋白)。虽然大量的生物和 已经获得了关于FBP的化学数据，这一分子的确切作用 蛋白质在淋球菌残留物存活和致病中的作用 投机性(2，3)。一个吸引人的假设是，FBP在 对铁的吸收至关重要的中心结合步骤。 在铁限制条件下生长的致病奈瑟氏菌。 因此，这种蛋白质的表达对生物体来说是至关重要的 获取生长所需的铁。显然，能够获得 生长必需的营养物质直接关系到一个人的能力 生物体在体内存活，并最终导致疾病。这个 目前的项目基于以下具体目标： 1.研究铁对FBP表达的调节作用。 2.检测FBP基因表达对淋球菌的可能影响 在小鼠小室模型中评估的发病机制(4)。 3.研究与铁的运输有关的分子事件 鉴定与铁转运和铁蛋白有关的其他蛋白质 利用率。 4.克隆氮藻中铁运输相关基因。 淋病。

The sites colonized by Neisseria gonorrhoeae within the human host are diverse and each site represents a unique niche with respect to nutrients, environmental factors and competing microorganisms. The growth environment has a marked effect on the metabolism and cellular composition of N. gonorrhoeae, and an altered cellular composition is often in the interaction of this microorganism with the human host (1). The overall goal of this project is to elucidate the specific mechanisms employed by N. gonorrhoeae for the assimilation of the essential nutrient iron. A majority of the parent project is focused on a study of the major iron binding protein in Neisseria commonly referred to as Fbp (ferric binding protein). While a great deal of biological and chemical data have been obtained on the Fbp, the precise role of this protein in the survival and pathogenesis of N. gonorrhoeae remains speculative (2,3). One attractive hypothesis is that Fbp functions in a central binding step that is essential for the assimilation of iron by the pathogenic Neisseria when grown under iron-restricted conditions. Expression of such a protein would thus be critical for the organism to obtain the iron necessary for growth. Clearly, the ability to obtain nutrients essential for growth is directly related to the ability of an organism to survive in vivo and ultimately to cause disease. The current project is based on the following specific aims: 1. To examine the regulation of Fbp expression by Fe. 2. To examine the possible influence of Fbp expression on gonococcal pathogenesis as assessed in a mouse chamber model (4). 3. To examine the molecular events involved in the transport of iron by identification of other proteins involved in iron transport and utilization. 4. To clone the genes involved in the transport of iron in N. gonorrhoeae.