IRON ASSIMILATION BY NEISSERIA GONORRHOEAE

淋病奈瑟菌对铁的同化

• 批准号: 6240366
• 负责人: Caroline A Genco
• 金额: $ 6.84万
• 依托单位: MOREHOUSE SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6240366
• 关键词: Neisseria gonorrhoeae; SDS polyacrylamide gel electrophoresis; binding proteins; biological transport; gene expression; growth media; host organism interaction; iron; iron metabolism; laboratory rat; membrane proteins; microorganism growth; molecular cloning; molecular pathology; polymerase chain reaction; protein purification; restriction mapping; southern blotting

The sites colonized by Neisseria gonorrhoeae within the human host are diverse and each site represents a unique niche with respect to nutrients, environmental factors and competing microorganisms. The growth environment has a marked effect on the metabolism and cellular composition of N. gonorrhoeae, and an altered cellular composition is often in the interaction of this microorganism with the human host (1). The overall goal of this project is to elucidate the specific mechanisms employed by N. gonorrhoeae for the assimilation of the essential nutrient iron. A majority of the parent project is focused on a study of the major iron binding protein in Neisseria commonly referred to as Fbp (ferric binding protein). While a great deal of biological and chemical data have been obtained on the Fbp, the precise role of this protein in the survival and pathogenesis of N. gonorrhoeae remains speculative (2,3). One attractive hypothesis is that Fbp functions in a central binding step that is essential for the assimilation of iron by the pathogenic Neisseria when grown under iron-restricted conditions. Expression of such a protein would thus be critical for the organism to obtain the iron necessary for growth. Clearly, the ability to obtain nutrients essential for growth is directly related to the ability of an organism to survive in vivo and ultimately to cause disease. The current project is based on the following specific aims: 1. To examine the regulation of Fbp expression by Fe. 2. To examine the possible influence of Fbp expression on gonococcal pathogenesis as assessed in a mouse chamber model (4). 3. To examine the molecular events involved in the transport of iron by identification of other proteins involved in iron transport and utilization. 4. To clone the genes involved in the transport of iron in N. gonorrhoeae.

淋病奈瑟菌在人类宿主体内的定植部位为 不同的，每个网站都代表了一个独特的利基方面， 营养物质、环境因素和竞争微生物。 的 生长环境对代谢和细胞生长有显著影响， 组成N.淋病，改变细胞组成， 通常在这种微生物与人类宿主的相互作用中（1）。 本项目的总体目标是阐明特定的机制 被N雇用。淋病的基本同化 营养铁 母项目的大部分内容都集中在研究上， 奈瑟氏菌中的主要铁结合蛋白， 铁结合蛋白（ferric binding protein）。 虽然大量的生物和 已经获得了关于Fbp的化学数据，这一点的确切作用 蛋白在N.淋病残留物 投机（2，3）。 一个有吸引力的假设是，Fbp的功能在一个 中心结合步骤，这是必不可少的同化铁， 在铁限制条件下生长的致病性奈瑟氏菌。 因此，这种蛋白质的表达对于生物体来说是至关重要的， 获得生长所需的铁。 很明显， 生长所必需的营养物质直接关系到 生物体在体内生存并最终导致疾病。 的 目前的项目基于以下具体目标： 1. 研究铁对Fbp表达的调控作用。 2. 检查Fbp表达对淋球菌的可能影响 在小鼠室模型中评估的发病机制（4）。 3. 为了研究铁转运中涉及的分子事件， 鉴定参与铁转运的其他蛋白质， 利用率 4. 克隆N. 淋病