EFFECTS OF 60 HZ ELECTROMAGNETIC FIELDS ON HUMAN CELLS

60 Hz 电磁场对人体细胞的影响

• 批准号: 6240185
• 负责人: Ann S Henderson
• 金额: $ 2.6万
• 依托单位: HUNTER COLLEGE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 1997-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6240185
• 关键词: biological signal transduction; calcium flux; cell line; electromagnetic radiation; gene expression; genetic promoter element; genetic transcription; human tissue; messenger RNA; molecular cloning; neoplastic transformation; protooncogene; radiation carcinogenesis; southern blotting; transcription factor; transfection; tumor promoters

This research will investigate the proposed link between exposure cells to extremely low frequency (elf) electromagnetic (EM) fields and malignancy. The major aim is to determine whether elf EM field exposure at 60 Hz can play a role in initiating, or augmenting neoplastic disease. The ramifications of this research have bearing on the general safety of exposure to EM fields in industrial and everyday life. Aim 1. To determine how the expression of cellular proto-oncogenes is altered in human cells exposed to elf EM fields by measurement of levels of mRNA transcripts and resulting proteins. Short exposures (1 to 60 min.) to defined elf EM fields will be used in initial experiments; further experiments will determine how the time course or other parameters of exposure affect mRNA levels. Aim 2. To investigate the role of signal transduction in cell response to elf EM fields; can a relation with signal transduction be made as predicted on the basis of previous studies? Transcripts of two transcription factors, c-myc and c-fos, are consistently increased in cells exposed to EM fields, and this response forms the basis for the proposed research. The experiments will determine if the time of response of the cell relative to induction of these transcripts following EM field exposure is consistent with other types of experiments where inductive agents are used. Second, each of the promotor region for these two genes will be transfected into human cells as a CAT construct to identify the specific sequence element(s) responsible for the induction. Preliminary experiments which have investigated the role of the "complete" c-myc promotor show that exposure of cells containing the transfected c-myc promotor to an elf EM field (B field =80muT; E field= 100 muV/m) for 20 minutes results in a significant increase in CAT expression.

这项研究将调查暴露细胞之间的拟议联系 至极低频（elf）电磁（EM）场， 恶性肿瘤 主要目的是确定是否有极低频电磁场暴露 在60 Hz的频率下，可以在启动或增强肿瘤疾病中起作用。 这项研究的结果关系到 在工业和日常生活中暴露于电磁场。 目标1. 到 确定细胞原癌基因的表达如何改变， 暴露于极低频电磁场的人细胞通过测量mRNA水平 转录物和产生的蛋白质。 短时间曝光（1至60分钟）到 定义的极低频电磁场将用于初始实验;进一步 实验将确定时间过程或其他参数如何 暴露影响mRNA水平。 目标二。 为了研究信号的作用 细胞对极低频电磁场响应的转导; 信号转导可以根据先前的预测进行 学习？ 两种转录因子c-myc和c-fos的转录本， 在暴露于电磁场的细胞中持续增加，这种反应 这是拟议研究的基础。 实验将 确定细胞相对于诱导的响应时间 这些转录后的EM场暴露是一致的，与其他 使用诱导剂的实验类型。 第二，这两个基因的每个启动子区将是 作为CAT构建体转染到人细胞中以鉴定特异性 负责诱导的序列元素。 初步 研究“完整的”c-myc的作用的实验 启动子显示含有转染c-myc的细胞的暴露 启动子与elf EM场（B场= 80 μ T; E场= 100 μ V/m）接触20 分钟导致CAT表达的显著增加。