DOES EXPOSURE OF HUMAN CELLS TO EM FIELDS CAUSE CANCER

人体细胞暴露于电磁场会导致癌症吗

• 批准号: 3254041
• 负责人: Ann S Henderson
• 金额: $ 46.56万
• 依托单位: HUNTER COLLEGE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-21 至 1996-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3254041
• 关键词: DNA replication; biological signal transduction; cell line; electrofocusing; electromagnetic radiation; fibroblasts; gel electrophoresis; genetic transcription; lymphocyte; messenger RNA; myelocyte; myeloid stem cell; neoplastic transformation; nucleic acid probes; phosphorylation; protooncogene; radiation carcinogenesis; simian virus 40; synchronous cell division; thymidine; tissue /cell culture

This research will investigate the proposed link between exposure of cells to extremely low frequency (ELF) electromagnetic (EM) fields and malignancy. We will determine if characteristics of neoplastic transformation and/or immortalization occur in human cells exposed to characterized ELF EM fields at 60 Hz. Aim 1. To determine if the expression of cellular proto-oncogenes is altered in human cells exposed to ELF EM fields by measurement of levels of mRNA transcripts and resulting proteins. Short exposures to defined ELF EM fields will be used initially; further experiments will determine how the time course or other parameters of exposure affect mRNA levels. Aim 2. To determine if cell divisional rates are changed by EM field exposure. Aim 3. To investigate the role of signal transduction in cell response to ELF EM fields; can a relation with signal transduction be made. as predicted on the basis of previous studies? Our major aim is to obtain adequate background information to ultimately allow us to experimentally test whether ELF EM field exposure can play a role in initiating, or augmenting neoplastic disease. The ramifications of this research, however, have bearing on the general safety of exposure to EM fields in industrial and everyday life.

这项研究将调查暴露于细胞之间的联系， 至极低频（ELF）电磁（EM）场， 恶性肿瘤 我们将确定肿瘤的特征 转化和/或永生化发生在暴露于 在60 Hz下表征ELF EM场。 目标1.以确定是否 在暴露的人细胞中细胞原癌基因的表达改变 通过测量mRNA转录物的水平， proteins. 最初将使用短时间暴露于定义的ELF EM场; 进一步的实验将确定时间进程或其他参数 影响mRNA水平。 目标二。为了确定细胞分裂 通过EM场暴露改变速率。 目标3。调查的作用 细胞对极低频电磁场反应中的信号转导; 进行信号转导。正如根据之前的研究所预测的那样？ 我们的主要目的是获得足够的背景资料， 允许我们通过实验测试ELF EM场暴露是否可以发挥 在启动或增强肿瘤性疾病中的作用。 的后果 然而，这项研究的结果与暴露的一般安全性有关， 到工业和日常生活中的电磁场。