SUPERCRITICAL FLUID EXTRACTION AND ELECTROCHEMICAL CHARACTERIZATION OF MYCOTOXINS

霉菌毒素的超临界流体萃取和电化学表征

• 批准号: 6240240
• 负责人: BENJAMIN T DUHART
• 金额: $ 3.37万
• 依托单位: UNIVERSITY OF ARKANSAS AT PINE BLUFF
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-01 至 1998-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6240240
• 关键词: aflatoxins; chemical transfer reaction; electrochemistry; food contamination; grain; high performance liquid chromatography; method development; mycotoxins; peanuts; physical separation

Mycotoxins are a class of compounds produced by certain strains of fungi or molds. These compounds may be toxic. Human health hazards and laboratory animals health hazards are possibly due to mycotoxin contaminated foods. The continuation project purposes are as follows: (1) To develop a method using supercritical fluid extraction (SFE) for extraction of aflatoxins from peanut butter and grains, (2) To continue to study cyclic voltammetry properties of mycotoxins - Aflatoxins B1, B2, G1, G2, aflatoxicol, and patulin using electrochemical reduction in various pH media, (3) To determine the number of electrons transferred and propose the possible organic mechanisms involved, and (4) To determine the percentage recoveries of spiked samples of aflatoxins. Undergraduate students knowledge will be enhanced by operating polarographic equipment HPLC instrument, and SFE equipment. Knowledge will also be gained with analytical skills, techniques and presentations.

真菌毒素是由某些真菌菌株产生的一类化合物。 或者霉菌。这些化合物可能是有毒的。危害人类健康和 实验动物的健康危害可能是由于霉菌毒素造成的。 受污染的食物。 续建项目的目的如下：(1)开发一个 超临界流体萃取法(SFE)提取三七总皂苷 花生酱和谷物中的黄曲霉毒素，(2)继续研究循环 霉菌毒素-黄曲霉毒素B1、B2、G1、G2、 黄曲霉毒素和棒曲霉毒素在不同pH值下的电化学还原 媒介，(3)确定转移的电子数并提出 可能涉及的有机机制，以及(4)确定 黄曲霉毒素加标样品的回收率。 通过操作提高本科生的知识水平 极谱设备、高效液相色谱仪和超临界流体萃取设备。知识 还将获得分析技能、技巧和演示文稿。