CORE--PATHOLOGY

核心--病理学

• 批准号: 6241609
• 负责人: BARBARA O MEYRICK
• 金额: $ 13.94万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-01 至 1997-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6241609
• 关键词: adult respiratory distress syndrome; biomedical facility; electron microscopy; immunocytochemistry; light microscopy; lung injury; morphology; pathology

Core D will provide the techniques and morphologic expertise for the studies proposed in this SCOR Program. Services include the personnel, supplies and facilities for performing light and electron microscopy, immunohistochemistry on tissue sections and bronchoalveolar lavage cells, autoradiography and in situ hybridization. Quantitative techniques will be applied tumor necrosis factor-a (TNF-a), interleukin-8, endothelin-1 (ET-1) and PGH synthase will be examined in BAL cells from patients with the Adult Respiratory Distress Syndrome (ARDS). In situ hybridization with probes to each peptide will also be used to demonstrate alterations each of the genes. Such data will indicate whether TNF antibodies and glutathione ethyl esters alter regulation of these peptides. In addition, we will provide routine histological examination of all available autopsy lungs and lung biopsy tissue from these patients. This will allow correlations between the structural changes in our animal models and patients with ARDS. In we will examine the morphological alterations in the lung following administration of TNF-a, PGH synthase and ET-1 in the presence and absence of a TNF-a antibody, a neutrophil adhesion blocker, NPC15669, and the glutathione ethyl ester, GSE. These data will allow correlation between structural, functional and biochemical changes and provide insights into the efficacy of each "protective" agent. We will use in situ hybridization to localize xanthine oxidase mRNA in ling and liver of sheep during endotoxemia. In project V. we will examine lung, liver and kidney tissues for evidence of injury following administration of various forms of liposomes, examination of DNA/liposome complexes by scanning microscopy, localization of liposomes in organs using a fluorescent marker (5,6- carboxyfluorescein), localization of cDNA's in tissue by in situ hybridization, visualization of reporter genes such as chloramphenicol acetyltransferase (CAT) and beta-galactosamine, as well as localization of PGH synthase by immunohistochemical techniques. The techniques and interpretative skills of this Core are crucial to the successful completion of this SCOR application.

核心D将提供技术和形态学专业知识， 在此SCOR计划中提出的研究。 服务包括人员， 进行光学和电子显微镜检查的用品和设施， 组织切片和支气管肺泡灌洗液细胞的免疫组织化学， 放射自显影和原位杂交。 定量技术将 应用肿瘤坏死因子-α（TNF-α）、白细胞介素-8、内皮素-1 将在来自患有以下疾病的患者的BAL细胞中检查ET-1和PGH合酶： 成人呼吸窘迫综合征（ARDS） 原位杂交 也将使用针对每种肽的探针来证明改变 每个基因。 这些数据将表明TNF抗体和 谷胱甘肽乙酯改变这些肽的调节。 在 此外，我们将提供常规组织学检查的所有 从这些病人的尸检肺和肺活检组织。 这 将使我们的动物的结构变化之间的相关性 模型和ARDS患者。 在我们将检查形态 在施用TNF-α、PGH合酶后肺中的改变 和ET-1在存在和不存在TNF-α抗体、嗜中性粒细胞 粘附阻滞剂，NPC 15669，和谷胱甘肽乙酯，GSE。 这些 数据将允许结构、功能和 生物化学的变化，并提供洞察每一个的功效 “保护”剂。 我们将用原位杂交技术 内毒素血症时绵羊肝脏和肝脏中黄嘌呤氧化酶mRNA的变化 在 我们将检查肺、肝和肾组织， 在施用各种形式的脂质体后的损伤， 通过扫描显微镜检查DNA/脂质体复合物， 使用荧光标记物（5，6- 羧基荧光素），通过原位荧光法在组织中定位cDNA 杂交、报告基因如氯霉素的可视化 乙酰转移酶（CAT）和β-半乳糖胺，以及定位 PGH合成酶的免疫组化研究。 的技术和 这一核心的解释技能是成功的关键， 完成SCOR应用程序。