CORE--PATHOLOGY

核心--病理学

• 批准号: 6109486
• 负责人: BARBARA O MEYRICK
• 金额: --
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6109486
• 关键词: adult respiratory distress syndrome; biomedical facility; electron microscopy; immunocytochemistry; light microscopy; lung injury; morphology; pathology

Core D will provide the techniques and morphologic expertise for the studies proposed in this SCOR Program. Services include the personnel, supplies and facilities for performing light and electron microscopy, immunohistochemistry on tissue sections and bronchoalveolar lavage cells, autoradiography and in situ hybridization. Quantitative techniques will be applied tumor necrosis factor-a (TNF-a), interleukin-8, endothelin-1 (ET-1) and PGH synthase will be examined in BAL cells from patients with the Adult Respiratory Distress Syndrome (ARDS). In situ hybridization with probes to each peptide will also be used to demonstrate alterations each of the genes. Such data will indicate whether TNF antibodies and glutathione ethyl esters alter regulation of these peptides. In addition, we will provide routine histological examination of all available autopsy lungs and lung biopsy tissue from these patients. This will allow correlations between the structural changes in our animal models and patients with ARDS. In we will examine the morphological alterations in the lung following administration of TNF-a, PGH synthase and ET-1 in the presence and absence of a TNF-a antibody, a neutrophil adhesion blocker, NPC15669, and the glutathione ethyl ester, GSE. These data will allow correlation between structural, functional and biochemical changes and provide insights into the efficacy of each "protective" agent. We will use in situ hybridization to localize xanthine oxidase mRNA in ling and liver of sheep during endotoxemia. In project V. we will examine lung, liver and kidney tissues for evidence of injury following administration of various forms of liposomes, examination of DNA/liposome complexes by scanning microscopy, localization of liposomes in organs using a fluorescent marker (5,6- carboxyfluorescein), localization of cDNA's in tissue by in situ hybridization, visualization of reporter genes such as chloramphenicol acetyltransferase (CAT) and beta-galactosamine, as well as localization of PGH synthase by immunohistochemical techniques. The techniques and interpretative skills of this Core are crucial to the successful completion of this SCOR application.

核心D将提供技术和形态专业知识 这项SCOR计划中提出的研究。服务包括人员， 用于进行光学和电子显微镜检查的用品和设施， 组织切片和支气管肺泡灌洗细胞的免疫组织化学， 放射自显影和原位杂交。量化技术将 应用肿瘤坏死因子-a、白介素8、内皮素-1 (ET-1)和PGH合成酶将被检测从患者的BAL细胞 成人呼吸窘迫综合征(ARDS)。原位杂交 也将使用对每种多肽的探针来证明变化 每一个基因。这些数据将表明肿瘤坏死因子抗体和 谷胱甘肽乙酯改变了这些多肽的调节。在……里面 此外，我们将为所有患者提供常规的组织学检查 这些病人的尸检肺和肺活检组织。这 将允许我们动物的结构变化之间的相互关系 模型和患有ARDS的患者。中，我们将检查形态 应用肿瘤坏死因子-α、前列腺素H合酶后肺组织的变化 和ET-1在存在和不存在肿瘤坏死因子-α抗体、中性粒细胞的情况下 黏附阻滞剂NPC15669和谷胱甘肽乙酯。这些 数据将允许结构、功能和 生化变化，并提供对每种药物疗效的洞察 “保护性”药剂。我们将使用原位杂交来定位 内毒素血症时绵羊肝脏和肝脏中黄嘌呤氧化酶mrna的表达。在……里面 项目V我们将检查肺、肝和肾组织以寻找证据 在注射各种形式的脂质体后的损伤， DNA/脂质体复合体的扫描显微镜检查， 利用荧光标记物(5，6-)对脂质体在器官中的定位 羧基荧光素)，原位定位组织中的cDNAs 氯霉素等报告基因的杂交、可视化 乙酰转移酶(CAT)和β-氨基半乳糖及其定位 用免疫组织化学技术检测PGH合成酶的表达。这些技巧和 这一核心的解释技能是成功的关键 完成这份SCOR申请。