ENDOMETRIUM EXTERIOR MEMBRANE INFECTION & PRETERM LABOR RHESUS MACAQUES MODEL

宫内感染是早产的重要原因。 我们以前已经证明，实验性羊膜腔内， 恒河猴感染导致羊水中的 体液（AF）、促炎细胞因子（TNF-α和IL-1）和 前列腺素（PGE 2和PGF 2“），然后早产， 交付. 然而，绒毛膜蜕膜感染在早产中的作用 劳动力不明确。 为了描述 绒毛膜蜕膜感染后的事件，我们长期使用 仪器恒河猴与定时妊娠，其中感染 通过接种低（S102 cfu; n=2）， 中等（103 - 104 cfu; n=4）或高（r105 cfu; n=3）接种物 B组（GBS组），将子宫内膜组织植入绒毛膜蜕膜间隙。 以下 接种后，2个低接种量组中无一例发生分娩，4个中等接种量组中有2例发生分娩 接种物和3只高接种物动物中的2只。 在4只动物中， 感染引产时，子宫收缩力增加 接种后21小时（14-30小时）。 子宫增大 3例GBS的羊膜内浸润先于收缩 4只动物平均12小时（6-24小时）。 AF促炎性 细胞因子和前列腺素浓度没有上升超过 在接种的5只动物中， 而不是导致劳动。 相比之下，AF的显著增加 4只分娩动物中均观察到细胞因子和胰头素 在GBS羊膜内恢复后，而不是之前， 液体浓度（pg/ml）事件TNF-“IL-1 PGE 2 PGF 2” 接种前50 <20 430 86分娩开始900 110 5，550 316 这些结果表明， 绒毛膜蜕膜接种可上升至羊膜腔， 在确定羊膜内感染后早产 包围 与此相反，绒毛膜蜕膜接种，不同时 羊膜腔感染未导致早产。

Intrauterine infection is an important cause of premature birth. We have previously demonstrated that experimental intraamniotic infection in rhesus monkeys leads to sequential increases in amniotic fluid (AF), proinflammatory cytokines (TNF-` and IL-1 ) and prostaglandins (PGE2 and PGF2`), followed by preterm labor and delivery. However, the role of choriodecidual infection in preterm labor is unclear. To characterize the pathophysiologic sequence of events following choriodecidual infection, we utilized chronically instrumented rhesus monkeys with timed gestations in which infection was established by inoculation of either a low (s102 cfu; n=2), moderate (103 -104 cfu; n=4), or high (r105 cfu; n=3) inoculum of Group B streptococcus (GBS) into the choriodecidual space. Following inoculation, labor occurred in none of 2 low inoculum, 2 of 4 moderate inoculum and in 2 of 3 high inoculum animals. In the 4 animals with infection-induced labor, increases in uterine contractility occurred 21 hours (14-30 hrs) after inoculation. Increases in uterine contractility were preceded by intraamniotic invasion by the GBS in 3 of 4 animals in an average of 12 hrs (6-24 hrs). AF proinflammatory cytokine and prostaglandin concentrations did not rise above pre-inoculation levels among those 5 animals in which inoculation did not result in labor. In contrast, significant increases in AF cytokines and prostaglandins were observed in the 4 laboring animals after, but not before, intraamniotic recovery of GBS Median Amniotic Fluid Concentrations (pg/ml) Event TNF-` IL-1 PGE2 PGF2` Pre-inoculation 50 <20 430 86 Onset of Labor 900 110 5,550 316 Delivery 1,320 844 15,754 1,474 These results demonstrate that choriodecidual inoculation may ascend to the amniotic cavity, and that following the establishment of intraamniotic infection preterm labor ensues. In contrast, choriodecidual inoculation without concurrent intraamniotic infection did not lead to preterm labor.