MAO/F CLONING AND PRODUCTION OF HIV-1 ENVELOPE PROTEINS

HIV的包膜蛋白被认为是艾滋病的一个重要靶点 疫苗设计 由于这种蛋白质存在于病毒颗粒上， 感染的细胞，这是合理的预测，免疫力提高到env 将是任何疫苗的精华部分，如果不是必不可少的。一个问题 不同的HIV进化枝有不同的包膜 可以被识别为与免疫系统不同的蛋白质。 此外，这些结构可以以不同的结构形式存在。 的 该合同的目标将是克隆各种特定的信封 HIV基因和工程表达载体， 在体外的给定的包膜蛋白。 接下来，这些包膜蛋白将 将其纯化，以便允许其施用给实验动物， 动物测试其免疫原性。 不同的形式，单体和 寡聚体，将被产生和纯化。 特殊的env基因 将由项目负责人确认。

The envelope protein of HIV is considered an integral target of AIDS vaccine design. Since this proteins is present on virus particles and infected cells, it is reasonable to predict that immunity raised to env will be a quintessential part of any vaccine, if not essential. A problem with this however, is that different HIV clades have different envelope proteins that can be recognized as different to the immune system. Also, the envelops can exist in different structural forms. The objectives of this contract will be to clone various specific envelope genes of HIV and engineer expression vectors capable of expressing the given envelope proteins in vitro. Next, these envelope proteins will be purified so as to permit their administration to experimental animals to test their immunogenicity. Different forms, monomeric and oligomeric, will be generated and purified. The particular env genes will be identified by the Project Officer.