PROTEIN X-RAY CRYSTALLOGRAPHY: HIV ENVELOPE PROTEIN

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。我们的实验是利用高能同步加速器光束线收集蛋白质晶体x射线衍射数据。多波长反常衍射（MAD）和/或单波长反常衍射（SAD）实验是在接近晶体中存在的重原子（通常是硒）吸收边缘的光束能量下进行的。我们所研究的大多数大分子都涉及一个或多个主要的生物学主题：细胞表面相互作用和信号转导、免疫反应相互作用、细胞对应激的反应、基因复制和转录、碳水化合物识别和氧气运输。另一些则被选为方法发展或一般结构原则分析的主题。我们目前正在研究的具体晶体分子包括CD4 t细胞共受体、t细胞受体、MHC分子、超级抗原、干细胞因子、成纤维细胞生长因子、胰岛素受体、淋巴细胞激酶、HIV包膜糖蛋白、FHIT、髓磷脂Po、N-钙粘蛋白、核糖核酸酶H、氨甲酰磷酸合成酶、UmuD、DnaK、链霉亲和素和血青素。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our experiment involves protein crystal x-ray diffraction data collection using the high energy synchrotron beamline. Multiple-wavelength anomalous diffraction (MAD) and/or single-wavelength anomalous diffraction (SAD) experiments are carried out at beam energies close to the absorption edge of heavy atoms present in the crystal, usually selenium. Most of the macromolecules that we have under study relate to one or more of a few main biological subjects: cell surface interactions and signal transduction, immune response interactions, cellular responses to stress, genetic replication and transcription, carbohydrate recognition, and oxygen transport. Others have been chosen as subject for methodology development or for the analysis of general structural principles. Specific crystalline molecules that we are presently studying include the CD4 T-cell co-receptors, T-cell receptors, MHC molecules, superantigens, stem cell factor, fibroblast growth factor, insulin receptor, lymphocyte kinase, HIV envelope glycoprotein, FHIT, myelin Po, N- cadherin, ribonuclease H, carbamyl phosphate synthetase, UmuD, DnaK, streptavidin, and hemocyanin.