PROTEIN X-RAY CRYSTALLOGRAPHY: HIV ENVELOPE PROTEIN

蛋白质 X 射线晶体学：HIV 包膜蛋白

• 批准号: 7369566
• 负责人: WAYNE A. HENDRICKSON
• 金额: $ 0.6万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7369566
• 关键词: PROTEIN; RAY; CRYSTALLOGRAPHY; HIV; ENVELOPE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our experiment involves protein crystal x-ray diffraction data collection using the high energy synchrotron beamline. Multiple-wavelength anomalous diffraction (MAD) and/or single-wavelength anomalous diffraction (SAD) experiments are carried out at beam energies close to the absorption edge of heavy atoms present in the crystal, usually selenium. Most of the macromolecules that we have under study relate to one or more of a few main biological subjects: cell surface interactions and signal transduction, immune response interactions, cellular responses to stress, genetic replication and transcription, carbohydrate recognition, and oxygen transport. Others have been chosen as subject for methodology development or for the analysis of general structural principles. Specific crystalline molecules that we are presently studying include the CD4 T-cell co-receptors, T-cell receptors, MHC molecules, superantigens, stem cell factor, fibroblast growth factor, insulin receptor, lymphocyte kinase, HIV envelope glycoprotein, FHIT, myelin Po, N- cadherin, ribonuclease H, carbamyl phosphate synthetase, UmuD, DnaK, streptavidin, and hemocyanin.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们的实验涉及使用高能同步辐射光束线的蛋白质晶体x射线衍射数据收集。多波长反常衍射（MAD）和/或单波长反常衍射（SAD）实验在接近晶体中存在的重原子（通常是硒）的吸收边缘的光束能量下进行。 我们正在研究的大部分大分子都与几个主要生物学主题中的一个或多个有关：细胞表面相互作用和信号转导，免疫反应相互作用，细胞对应激的反应，遗传复制和转录，碳水化合物识别和氧运输。另一些则被选为方法学发展或一般结构原则分析的主题。我们目前正在研究的特定晶体分子包括CD 4 T细胞共受体、T细胞受体、MHC分子、超抗原、干细胞因子、成纤维细胞生长因子、胰岛素受体、淋巴细胞激酶、HIV包膜糖蛋白、FHIT、髓磷脂Po、N-钙粘蛋白、核糖核酸酶H、氨甲酰磷酸合成酶、UmuD、DnaK、链霉亲和素和血蓝蛋白。