PROTEIN X-RAY CRYSTALLOGRAPHY: HIV ENVELOPE PROTEIN

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我们的实验包括利用高能同步加速器光束线采集蛋白质晶体的X射线衍射数据。多波长反常衍射(MAD)和/或单波长反常衍射(SAD)实验是在接近晶体中重原子(通常是Se)吸收边的束流能量下进行的。我们正在研究的大多数大分子与几个主要生物学主题中的一个或多个有关：细胞表面相互作用和信号转导、免疫反应相互作用、细胞对应激的反应、遗传复制和转录、碳水化合物识别和氧运输。另一些则被选为制定方法或分析一般结构原则的对象。我们目前正在研究的特定晶体分子包括CD4T细胞共受体、T细胞受体、MHC分子、超抗原、干细胞因子、成纤维细胞生长因子、胰岛素受体、淋巴细胞酶、HIV包膜糖蛋白、FHIT、髓磷脂、N-钙粘素、核糖核酸酶H、氨基甲酰磷酸合成酶、UmuD、DNAK、链霉亲和素和血蓝蛋白。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our experiment involves protein crystal x-ray diffraction data collection using the high energy synchrotron beamline. Multiple-wavelength anomalous diffraction (MAD) and/or single-wavelength anomalous diffraction (SAD) experiments are carried out at beam energies close to the absorption edge of heavy atoms present in the crystal, usually selenium. Most of the macromolecules that we have under study relate to one or more of a few main biological subjects: cell surface interactions and signal transduction, immune response interactions, cellular responses to stress, genetic replication and transcription, carbohydrate recognition, and oxygen transport. Others have been chosen as subject for methodology development or for the analysis of general structural principles. Specific crystalline molecules that we are presently studying include the CD4 T-cell co-receptors, T-cell receptors, MHC molecules, superantigens, stem cell factor, fibroblast growth factor, insulin receptor, lymphocyte kinase, HIV envelope glycoprotein, FHIT, myelin Po, N- cadherin, ribonuclease H, carbamyl phosphate synthetase, UmuD, DnaK, streptavidin, and hemocyanin.