Classic and temporal mixture synergism in terrestrial ecosystems: Prevalence, mechanisms and impacts

陆地生态系统中的经典和时间混合协同作用：普遍性、机制和影响

• 批准号: NE/S000240/1
• 负责人: Jacob Bundy
• 金额: $ 40.86万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FS000240%2F1
• 关键词: Classic; temporal; mixture; synergism; terrestrial

Invertebrate species living above and below ground are central to terrestrial food webs and key contributors to carbon cycling, soil fertility and pest control. Many of these important species are highly vulnerable to chemical pollution. The range of chemicals these species are regularly exposed to is becoming increasingly complex. For example, farmers now use 50% more types of pesticide on arable crops than they did 15 years ago and an ever-increasing diversity of chemicals enter ecosystems from our domestic and industrial wastes. A challenge for chemical producers, users and regulators is to find ways to maximise the benefits of chemical use, while minimising any negative effects. Scientific research to support better 'ecological risk assessment' of chemicals is central to meeting this challenge. Many of the chemicals we use today come from new, less well studied, compound classes that can affect biological processes, in diverse ways, in different species. Our current lack of knowledge about these chemicals makes their ecological effects difficult to assess. Things become more complicated when we realise that pollutants almost always occur as mixtures. If we want to properly to address and avoid unwanted chemical impacts, we need to better understand and take account of chemical mixtures. The most commonly used way to predict the likely effects of pollutant mixtures on invertebrates and ecosystems assumes that chemicals do not interact with each other and that, therefore, their toxicities can be added together. This relatively simple 'additive' approach has been shown to work most of the time. However, for a substantial proportion of mixtures (up to 20% depending on chemical classes included), the observed effects are worse than expected based on addition. Where such 'synergy' occurs, environmental protection policies for mixtures based on additivity will underestimate actual effects (see Fig. 1, Case for Support). Clearly this is a problem. To address it, we need to identify interactive chemical mixtures and predict the most likely causes of synergy. In turn, this requires us to understand how the mechanisms of toxicity of different chemicals in a mixture interplay with the different biochemical, physiological and ecological traits of exposed species to cause synergy. The main aim of this project is to gain and apply this knowledge. Our own research has identified some chemical mixtures that are more likely to show synergy, with higher levels of toxicity to exposed invertebrates. For example, where: (a) a chemical affects the way that another is detoxified or activated; or (b) a chemical increases the biological uptake of another chemical; or (c) prior exposure to a chemical changes the biological toxicity of another chemical, depending on the timing of exposure. However, we are very far from understanding all cases. Thus, this project aims to transform our ability to identify, quantify and predict the potential for synergy in common terrestrial pollution scenarios (agrochemical use, waste inputs). Working with partner agencies, we will identify potentially synergistic chemical pollutant mixtures, relevant to terrestrial ecosystems, and conduct experiments to test their effects on a range of invertebrate species. When we observe synergy in one species, other species will be tested to discover if this is a general effect. Biochemical and genetic methods will be used to identify mechanisms of toxicity and species traits associated with synergism, integrating this information to develop models and new predictive tools. To ensure the effects we see in the laboratory are relevant to the field, we will conduct studies in outdoor systems to test for the presence of synergism in natural communities. Ultimately, we will use our findings to produce a POSTnote 'White paper' detailing how future risk assessment policies can explicitly consider synergism to support environmental protection.

生活在地上和地下的无脊椎动物物种是陆地食物网的核心，也是碳循环、土壤肥力和害虫防治的关键贡献者。许多重要物种极易受到化学污染。这些物种经常接触的化学物质范围变得越来越复杂。例如，现在农民在耕种作物上使用的农药种类比 15 年前多了 50%，并且越来越多的化学品从我们的家庭和工业废物进入生态系统。化学品生产商、用户和监管机构面临的挑战是找到方法最大限度地发挥化学品使用的好处，同时最大限度地减少任何负面影响。支持更好的化学品“生态风险评估”的科学研究对于应对这一挑战至关重要。我们今天使用的许多化学品来自新的、研究较少的化合物类别，它们可以以多种方式影响不同物种的生物过程。我们目前对这些化学物质缺乏了解，使得它们的生态影响难以评估。当我们意识到污染物几乎总是以混合物的形式出现时，事情变得更加复杂。如果我们想正确解决和避免不必要的化学影响，我们需要更好地了解和考虑化学混合物。预测污染物混合物对无脊椎动物和生态系统可能产生的影响的最常用方法假设化学物质不会相互作用，因此它们的毒性可以加在一起。这种相对简单的“附加”方法已被证明在大多数情况下都是有效的。然而，对于很大比例的混合物（高达 20%，具体取决于所含化学类别），观察到的效果比基于添加的预期效果更差。当这种“协同作用”发生时，基于可加性的混合物环境保护政策将低估实际效果（见图1，支持案例）。显然这是一个问题。为了解决这个问题，我们需要识别相互作用的化学混合物并预测最可能产生协同作用的原因。反过来，这要求我们了解混合物中不同化学物质的毒性机制如何与暴露物种的不同生化、生理和生态特征相互作用，从而产生协同作用。该项目的主要目的是获得并应用这些知识。我们自己的研究已经发现一些化学混合物更有可能表现出协同作用，对暴露的无脊椎动物具有更高的毒性。例如，如果： (a) 一种化学物质影响另一种化学物质的解毒或激活方式； (b) 一种化学品增加另一种化学品的生物吸收； (c) 之前接触某种化学品会改变另一种化学品的生物毒性，具体取决于接触时间。然而，我们距离理解所有案例还很远。因此，该项目旨在提高我们识别、量化和预测常见陆地污染情景（农用化学品使用、废物输入）协同潜力​​的能力。我们将与伙伴机构合作，确定与陆地生态系统相关的潜在协同化学污染物混合物，并进行实验来测试它们对一系列无脊椎动物物种的影响。当我们观察一个物种的协同作用时，将对其他物种进行测试以发现这是否是普遍效应。生化和遗传学方法将用于确定与协同作用相关的毒性机制和物种特征，整合这些信息来开发模型和新的预测工具。为了确保我们在实验室中看到的效果与现场相关，我们将在室外系统中进行研究，以测试自然群落中是否存在协同作用。最终，我们将利用我们的发现来制作一份 POSTnote“白皮书”，详细说明未来的风险评估政策如何明确考虑协同作用以支持环境保护。

项目成果

Avoiding the Misuse of Pathway Analysis Tools in Environmental Metabolomics.

• DOI: 10.1021/acs.est.2c05588
• 发表时间: 2022-10-18
• 期刊: ENVIRONMENTAL SCIENCE & TECHNOLOGY
• 影响因子: 11.4
• 作者: Wieder, Cecilia;Bundy, Jacob G.;Frainay, Clement;Poupin, Nathalie;Rodriguez-Mier, Pablo;Vinson, Florence;Cooke, Juliette;Lai, Rachel P. J.;Jourdan, Fabien;Ebbels, Timothy M. D.
• 通讯作者: Ebbels, Timothy M. D.

Ion-Pairing Chromatography and Amine Derivatization Provide Complementary Approaches for the Targeted LC-MS Analysis of the Polar Metabolome.

• DOI: 10.1021/acs.jproteome.2c00030
• 发表时间: 2022-06-03
• 期刊: JOURNAL OF PROTEOME RESEARCH
• 影响因子: 4.4
• 作者: Sagi-Kiss, Virag;Li, Yufeng;Carey, Matthew R.;Grover, Sarah J.;Siems, Karsten;Cirulli, Francesca;Berry, Alessandra;Musillo, Chiara;Wilson, Ian D.;Want, Elizabeth J.;Bundy, Jacob G.
• 通讯作者: Bundy, Jacob G.