NEGATIVE REGULATORY ELEMENT OF HAMSTER MUC 1 PROMOTER

仓鼠 MUC 1 启动子的负调控元件

• 批准号: 6351583
• 负责人: KWANG CHUL KIM
• 金额: $ 17.53万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-01 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6351583
• 关键词: DNA binding protein; affinity chromatography; breast neoplasms; cell differentiation; cell growth regulation; gel mobility shift assay; gene expression; gene induction /repression; genetic library; genetic promoter element; genetic regulation; genetic regulatory element; goblet cells; hamsters; messenger RNA; molecular cloning; mucins; neoplastic cell; northern blottings; nucleotides; protein binding; respiratory epithelium; tissue /cell culture; transcription factor; transfection /expression vector

DESCRIPTION (Adapted from the Applicant's Abstract): MUC 1 is a transmembrane cell surface mucin best known for its upregulation in breast cancer. The PI observes that its expression correlates with goblet cell differentiation in hamster tracheal epithelial cells in vitro. His preliminary promoter analysis of the hamster Muc 1 gene cloned in his lab reveals the presence of a silencer (P-NRE) not previously described. He proposes conventional methods to (1) deduce the nucleotides comprising the silencer, (2) identify the protein binding to the silencer, (3) clone this protein and (4) perform studies to determine if and how the protein-DNA interaction mediates Muc 1 upregulation during goblet cell differentiation in vitro.

描述（改编自申请人的摘要）：MUC 1是跨膜的， 细胞表面粘蛋白在乳腺癌中以其上调而闻名。的PI 观察到它的表达与杯状细胞分化相关， 仓鼠气管上皮细胞体外培养。他的初步启动子分析 在他的实验室中克隆的仓鼠Muc 1基因揭示了沉默者的存在 （P-NRE）先前未描述的。他提出了常规方法来（1） 推导出组成沉默子的核苷酸，（2）鉴定蛋白质 结合沉默子，（3）克隆这种蛋白质，（4）进行研究， 确定蛋白质-DNA相互作用是否以及如何介导Muc 1上调 杯状细胞体外分化过程中。