NEGATIVE REGULATORY ELEMENT OF HAMSTER MUC 1 PROMOTER

描述(改编自申请人的摘要)：MUC 1是一种跨膜 细胞表面粘蛋白，以其在乳腺癌中的上调而闻名。《少年派》 观察到其表达与杯状细胞分化有关。 仓鼠气管上皮细胞的体外培养。他对启动子的初步分析 在他的实验室中克隆的仓鼠MUC1基因的一部分揭示了沉默的存在 (P-NRE)之前未描述的。他提出了传统的方法来(1) 推断组成消音器的核苷酸，(2)鉴定蛋白质 与消音器结合，(3)克隆该蛋白质，以及(4)进行研究以 确定蛋白质-DNA相互作用是否以及如何介导MUC1上调 在体外杯状细胞分化过程中。

DESCRIPTION (Adapted from the Applicant's Abstract): MUC 1 is a transmembrane cell surface mucin best known for its upregulation in breast cancer. The PI observes that its expression correlates with goblet cell differentiation in hamster tracheal epithelial cells in vitro. His preliminary promoter analysis of the hamster Muc 1 gene cloned in his lab reveals the presence of a silencer (P-NRE) not previously described. He proposes conventional methods to (1) deduce the nucleotides comprising the silencer, (2) identify the protein binding to the silencer, (3) clone this protein and (4) perform studies to determine if and how the protein-DNA interaction mediates Muc 1 upregulation during goblet cell differentiation in vitro.