IMMUNE MECHANISMS OF MIDDLE EAR DISEASE

中耳疾病的免疫机制

• 批准号: 6115527
• 负责人: PHILIP FIREMAN
• 金额: $ 2.06万
• 依托单位: CHILDREN'S HOSP PITTSBURGH/UPMC HLTH SYS
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6115527
• 关键词: clinical research; cytokine; disease /disorder proneness /risk; eustachian tube; histamine; human subject; hypersensitivity; immunoglobulin E; immunopathology; influenzavirus A; middle ear disorder; otitis media; pathologic process; rhinitis; rhinovirus

This study explores the pathogenesis of otitis media with effusion (OME) and assesses the contribution of the various risk factors including infection, especially viral, allergy and immune competence. Experimental URI using rhinovirus (RV-39) and influenza A virus (IFA) were induced in normal susceptible human volunteers with and without a history of allergic rhinitis. Eustachian tube dysfunction was induced in 70 to 80% of infected subjects for both viruses with OME in 3% of RV-39 and 20% of IFA infected subjects. Enhanced serum IgE synthesis and WBC histamine release was documented in allergic but not in non-allergic subjects; whereas both groups had enhanced cytokines (IL-6, 8 and 10) in nasal secretions. The effect of Respiratory Synctial Virus (RSV) on upper and lower airway function is now being explored. Two cohorts have been experimentally inoculated and data is currently being analyzed. Preliminary results from one cohort shows that RSV provoked upper airway pathophysiology which extended to otologic parameters, in experimentally infected subjects.

本研究探讨渗出性中耳炎(OME)的发病机制，并评估各种危险因素的贡献，包括感染，特别是病毒、过敏和免疫功能。使用鼻病毒(RV-39)和甲型流感病毒(IFA)在有和没有过敏性鼻炎病史的正常易感人类志愿者中诱导实验性URI。同时感染OME的受试者中，70%至80%的受试者会出现咽鼓管功能障碍，3%的RV-39和20%的IFA感染者会出现咽鼓管功能障碍。变应性鼻炎患者的血清IgE合成和WBC组胺释放增加，而非过敏性鼻炎患者未见；而两组患者鼻分泌物中的细胞因子(IL-6、8和10)均升高。呼吸道合胞病毒(RSV)对上、下呼吸道功能的影响目前正在研究中。已经对两个队列进行了实验性接种，目前正在分析数据。一个队列的初步结果显示，在实验感染的受试者中，RSV引起了上呼吸道的病理生理学，并延伸到耳科参数。