IMMUNE MECHANISMS OF MIDDLE EAR DISEASE

本研究探讨了渗出性中耳炎（OME）的发病机制，并评估了各种危险因素，包括感染，特别是病毒，过敏和免疫能力的贡献。 在有和没有变应性鼻炎病史的正常易感人类志愿者中诱导使用鼻病毒（RV-39）和甲型流感病毒（IFA）的实验性URI。 两种病毒均在70 - 80%的感染受试者中诱导咽鼓管功能障碍，RV-39感染受试者中有3%和IFA感染受试者中有20%出现OME。 增强血清IgE合成和白细胞组胺释放记录在过敏，但不是在非过敏性受试者，而这两个群体有增强的细胞因子（IL-6，8和10）的鼻分泌物。呼吸道合胞病毒（RSV）对上下气道功能的影响正在研究中。 已对两个队列进行了实验性接种，目前正在分析数据。 一个队列的初步结果显示，RSV引起实验感染受试者的上呼吸道病理生理学，其扩展到耳科参数。

This study explores the pathogenesis of otitis media with effusion (OME) and assesses the contribution of the various risk factors including infection, especially viral, allergy and immune competence. Experimental URI using rhinovirus (RV-39) and influenza A virus (IFA) were induced in normal susceptible human volunteers with and without a history of allergic rhinitis. Eustachian tube dysfunction was induced in 70 to 80% of infected subjects for both viruses with OME in 3% of RV-39 and 20% of IFA infected subjects. Enhanced serum IgE synthesis and WBC histamine release was documented in allergic but not in non-allergic subjects; whereas both groups had enhanced cytokines (IL-6, 8 and 10) in nasal secretions. The effect of Respiratory Synctial Virus (RSV) on upper and lower airway function is now being explored. Two cohorts have been experimentally inoculated and data is currently being analyzed. Preliminary results from one cohort shows that RSV provoked upper airway pathophysiology which extended to otologic parameters, in experimentally infected subjects.