CORE--VIRUS/PRIMARY CELL FACILITY

病毒-原代细胞核心的目的是为基于病毒和原代细胞的研究提供全面支持，以提高宾夕法尼亚大学/Wistar/CHOP社区内已建立的艾滋病毒研究人员的生产力和效率，促进新研究人员进入艾滋病研究领域，并促进这些研究人员之间的科学合作。该中心将提供：(1)一个受到严格监督的设施，供无法进入个别密闭设施或需要比现有密闭水平更高的密闭设施的调查人员进行3级生物安全培训和密闭实验；(2) HIV-1原型毒株、突变病毒毒株和表征良好的代表不同发病机制的原代病毒分离株的中央储存库；(3)原发HIV-1的分离、滴定和鉴定服务；(4)一个专门的原代细胞项目，将促进与艾滋病毒研究相关的专门的原代细胞类型的利用（通过为试点实验提供细胞，为分离专门细胞类型的调查提供培训，并促进协作互动），并通过为艾滋病毒研究提供具有明确基因型的血液单核细胞（CCR5, CCR2b, SDF-1）。我们期望这一核心将加强现有艾滋病毒研究人员之间的合作和生产力，并促进非艾滋病毒研究人员进入该领域，同时提供一种加强实验室生物安全的机制。

The purpose of the Virus-Primary Cell Core is to provide comprehensive support for virus and primary cell-based studies in order to enhance the productivity and efficiency of established HIV investigators within the Penn/Wistar/CHOP community, facilitate the entrance of new investigators into the field of AIDS research, and promote scientific collaborations among these investigators. The core will provide: (1) a carefully supervised facility for Biosafety Level 3 training and for the performance of containment experiments by investigators without access to individual containment facilities or who require a level of containment greater than currently available; (2) a central repository of prototype HIV-1 strains, mutant virus strains, and well-characterized primary virus isolates representing different aspects of pathogenesis; (3) a service for primary HIV-1 isolation, titration and characterization, and; (4) a specialized primary cell program that will facilitate utilization of specialized primary cell types relevant to HIV studies (by providing cells for pilot experiments, training of investigations in the isolation of specialized cell types, and facilitating collaborative interaction) and by providing blood mononuclear cells with defined genotypes important for HIV studies (CCR5, CCR2b, SDF-1). It is our expectation that this core will enhance collaborations and productivity among existing HIV investigators and facilitate the entrance of non-HIV investigators into the field while providing a mechanisms to enhance laboratory biosafety.