Viral & Molecular

病毒性的

• 批准号: 7684970
• 负责人: Ronald G Collman
• 金额: $ 27.21万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-25 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7684970
• 关键词: AIDS prevention; AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Adoption; Area; Arts; Biological Assay; Biometry; Botswana; Cells; Clinical; Cloning; Collaborations; Consultations; Custom; Development; Educational Curriculum; Enzyme-Linked Immunosorbent Assay; Feedback; Funding; Genes; Genomics; Genotype; Goals; HIV; HIV Core Protein p24; HIV Infections; HIV-1; Human Resources; International; Laboratories; Laboratory Personnel; Leadership; Maintenance; Mediator of activation protein; Molecular; Molecular Biology; Molecular Virology; Mycoplasma; Neuropathogenesis; Operative Surgical Procedures; Pathogenesis; Patient Isolation; Phenotype; Procedures; Reagent; Research; Research Personnel; Resistance; SIV; Sampling; Seeds; Services; Specimen; Techniques; Technology; Testing; Training; Training Support; Training and Education; Translational Research; Vaccine Design; Viral; Virion; Virus; Work; data management; density; drug of abuse; flexibility; gene therapy; inhibitor/antagonist; innovation; member; multidisciplinary; mutant; new technology; novel; novel strategies; programs; prototype; quality assurance; repository; response; virology

The Viral/Molecular Core (Core D) provides reagents, facilities, lab services, consultation & training in support of innovative, multidisciplinary and translational HIV/AIDS research on the Penn/CHOP/Wistar campus with the broad goal of advancing collaborative research in the pathogenesis, treatment, and prevention of HIV/AIDS, serving CFAR investigators from multiple programs. The specific Core activities supporting this goal are to provide: ¿ Virology services including a repository of HIV-1 prototype, primary and mutant strains; virus isolation from patient samples; amplification of virus working stocks; structurally intact inactivated virions; HIV p24 and SIV p27 ELISA assay; and other viral services. ¿ Molecular services including support for realtime qPCR along with reagents, optimized primer-probe sets and custom primer/probe development; viral genotyping or cloning from clinical specimens; molecular virology support for translational research programs; and in the next cycle a new high density pyrosequencing service. ¿ Education, training, consultation and leadership in molecular virology activities including training in BSL3 practices for CFAR members lab personnel; training and consultation in virological and molecular techniques for CFAR investigator personnel; facilitating the dissemination and adoption of new technologies; and collaboration with and support of other Cores and campus programs in their HIV/AIDS research-related educational efforts. During the current funding cycle the Core provided support to 46 CFAR investigators, contributing to novel gene therapy approaches to HIV infection; better understanding of integration and establishment of latency; new approaches to vaccine design; advances in viral entry and resistance to entry inhibitors; effects of neuroimmune mediators & drugs of abuse; HIV neuropathogenesis; and other areas. The V/M Core has evolved significantly over the past 9 years in response to CFAR member needs and user feedback, levels of utilization, and internal & external advisory input, and collaborates closely with other CFAR Cores in support of these goals. The Core has been directed since its inception by Drs. Ronald Collman (director) and Luis Montaner (co-director) and, going forward, will be joined by Dr. F. (Rick) Bushman (co-director) to supervise the introduction and dissemination of new molecular sequencing technologies to CFAR investigators.

病毒/分子核心(核心D)提供试剂、设施、实验室服务、咨询和培训 支持宾夕法尼亚大学/CHOP/Wistar的创新、多学科和翻译性艾滋病毒/艾滋病研究 园区的广泛目标是推进发病机制、治疗和治疗方面的合作研究 预防艾滋病毒/艾滋病，为来自多个项目的CFAR调查人员提供服务。具体的核心活动 支持这一目标的目的是： 病毒学服务，包括艾滋病毒-1原型、初级和变异毒株的资料库；病毒分离 从患者样本中；病毒工作样本的放大；结构完整的灭活病毒粒子；艾滋病毒p24和 SIV p27酶联免疫吸附试验；以及其他病毒服务。 ？分子服务，包括支持实时定量聚合酶链式反应和试剂、优化的引物-探针 套装和定制引物/探针开发；病毒基因分型或临床标本克隆；分子 为翻译研究项目提供病毒学支持；并在下一个周期中实现新的高密度 焦磷酸测序服务。 ·分子病毒学活动方面的教育、培训、咨询和领导，包括培训 CFAR成员实验室人员的BSL3实践；病毒学和分子方面的培训和咨询 为CFAR调查员提供技术；促进新技术的传播和采用； 以及在与艾滋病毒/艾滋病相关的研究中与其他核心和校园方案合作并提供支持 教育方面的努力。 在本供资周期内，核心向46名CFAR调查员提供了支助，为 艾滋病毒感染的新基因治疗方法；更好地理解整合和建立 潜伏期；疫苗设计的新方法；病毒进入和抵抗进入抑制剂的进展；效果 神经免疫介质和滥用药物；艾滋病毒神经发病机制；以及其他领域。V/M核心具有 根据CFAR成员的需求和用户反馈，在过去9年中显著发展， 利用情况以及内部和外部咨询意见，并与其他CFAR核心密切合作，提供支持 这些目标。《核心》从一开始就由罗纳德·科尔曼博士(导演)和路易斯博士执导 蒙塔纳(联席导演)，展望未来，F.(里克)布什曼博士(联席导演)将加入监督 向CFAR调查人员介绍和传播新的分子测序技术。