Viral & Molecular

病毒性的

• 批准号: 7684970
• 负责人: Ronald G Collman
• 金额: $ 27.21万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-25 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7684970
• 关键词: AIDS prevention; AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Adoption; Area; Arts; Biological Assay; Biometry; Botswana; Cells; Clinical; Cloning; Collaborations; Consultations; Custom; Development; Educational Curriculum; Enzyme-Linked Immunosorbent Assay; Feedback; Funding; Genes; Genomics; Genotype; Goals; HIV; HIV Core Protein p24; HIV Infections; HIV-1; Human Resources; International; Laboratories; Laboratory Personnel; Leadership; Maintenance; Mediator of activation protein; Molecular; Molecular Biology; Molecular Virology; Mycoplasma; Neuropathogenesis; Operative Surgical Procedures; Pathogenesis; Patient Isolation; Phenotype; Procedures; Reagent; Research; Research Personnel; Resistance; SIV; Sampling; Seeds; Services; Specimen; Techniques; Technology; Testing; Training; Training Support; Training and Education; Translational Research; Vaccine Design; Viral; Virion; Virus; Work; data management; density; drug of abuse; flexibility; gene therapy; inhibitor/antagonist; innovation; member; multidisciplinary; mutant; new technology; novel; novel strategies; programs; prototype; quality assurance; repository; response; virology

The Viral/Molecular Core (Core D) provides reagents, facilities, lab services, consultation & training in support of innovative, multidisciplinary and translational HIV/AIDS research on the Penn/CHOP/Wistar campus with the broad goal of advancing collaborative research in the pathogenesis, treatment, and prevention of HIV/AIDS, serving CFAR investigators from multiple programs. The specific Core activities supporting this goal are to provide: ¿ Virology services including a repository of HIV-1 prototype, primary and mutant strains; virus isolation from patient samples; amplification of virus working stocks; structurally intact inactivated virions; HIV p24 and SIV p27 ELISA assay; and other viral services. ¿ Molecular services including support for realtime qPCR along with reagents, optimized primer-probe sets and custom primer/probe development; viral genotyping or cloning from clinical specimens; molecular virology support for translational research programs; and in the next cycle a new high density pyrosequencing service. ¿ Education, training, consultation and leadership in molecular virology activities including training in BSL3 practices for CFAR members lab personnel; training and consultation in virological and molecular techniques for CFAR investigator personnel; facilitating the dissemination and adoption of new technologies; and collaboration with and support of other Cores and campus programs in their HIV/AIDS research-related educational efforts. During the current funding cycle the Core provided support to 46 CFAR investigators, contributing to novel gene therapy approaches to HIV infection; better understanding of integration and establishment of latency; new approaches to vaccine design; advances in viral entry and resistance to entry inhibitors; effects of neuroimmune mediators & drugs of abuse; HIV neuropathogenesis; and other areas. The V/M Core has evolved significantly over the past 9 years in response to CFAR member needs and user feedback, levels of utilization, and internal & external advisory input, and collaborates closely with other CFAR Cores in support of these goals. The Core has been directed since its inception by Drs. Ronald Collman (director) and Luis Montaner (co-director) and, going forward, will be joined by Dr. F. (Rick) Bushman (co-director) to supervise the introduction and dissemination of new molecular sequencing technologies to CFAR investigators.

病毒/分子核（核心D）提供试剂，设施，实验室服务，咨询和培训 支持宾夕法尼亚州/CHOP/WISTAR的创新，多学科和翻译艾滋病毒/艾滋病研究 校园的广泛目标是推进发病机理，治疗和 预防艾滋病毒/艾滋病，为CFAR调查人员提供多个计划的调查人员。特定的核心活动 支持此目标是提供： »病毒学服务，包括HIV-1原型，原发性和突变菌株的存储库；病毒隔离 从患者样本中；扩增病毒工作库存；结构完整的灭活病毒；艾滋病毒P24和 SIV P27 ELISA分析；和其他病毒服务。 »分子服务，包括对实时QPCR的支持以及试剂，优化的引物探针 集合和自定义底漆/探针开发；临床标本的病毒基因分型或克隆；分子 对翻译研究计划的病毒学支持；在下一个周期中，新的高密度 pyrosequencing服务。 �分子病毒学活动的教育，培训，咨询和领导才能，包括培训 CFAR成员实验室人员的BSL3实践；病毒学和分子的培训和咨询 CFAR调查员人员的技术；促进新技术的传播和采用； 以及与其他核心和校园计划在其艾滋病毒/艾滋病研究中与其他核心和校园计划的合作 教育工作。 在当前的融资周期中，核心为46名CFAR调查人员提供了支持，这有助于 新型基因治疗方法用于HIV感染；更好地理解整合和建立 潜伏期；疫苗设计的新方法；病毒进入和对进入抑制剂的抵抗力的进步；效果 神经免疫介质和虐待药物； HIV神经病发生；和其他领域。 v/m核心有 在过去的9年中，由于CFAR成员需求和用户反馈，级别 利用以及内部和外部咨询输入，并与其他CFAR内核紧密合作以支持 这些目标。自从Drs成立以来，该核心已被指导。罗纳德·科尔曼（Ronald Collman）（导演）和路易斯（Luis） Montaner（联合导演），并将与F.（Rick）Bushman博士（联合导演）一起监督 新分子测序技术的引入和传播给CFAR研究者。