Viral & Molecular

病毒性的

• 批准号: 7684970
• 负责人: Ronald G Collman
• 金额: $ 27.21万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-25 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7684970
• 关键词: AIDS prevention; AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Adoption; Area; Arts; Biological Assay; Biometry; Botswana; Cells; Clinical; Cloning; Collaborations; Consultations; Custom; Development; Educational Curriculum; Enzyme-Linked Immunosorbent Assay; Feedback; Funding; Genes; Genomics; Genotype; Goals; HIV; HIV Core Protein p24; HIV Infections; HIV-1; Human Resources; International; Laboratories; Laboratory Personnel; Leadership; Maintenance; Mediator of activation protein; Molecular; Molecular Biology; Molecular Virology; Mycoplasma; Neuropathogenesis; Operative Surgical Procedures; Pathogenesis; Patient Isolation; Phenotype; Procedures; Reagent; Research; Research Personnel; Resistance; SIV; Sampling; Seeds; Services; Specimen; Techniques; Technology; Testing; Training; Training Support; Training and Education; Translational Research; Vaccine Design; Viral; Virion; Virus; Work; data management; density; drug of abuse; flexibility; gene therapy; inhibitor/antagonist; innovation; member; multidisciplinary; mutant; new technology; novel; novel strategies; programs; prototype; quality assurance; repository; response; virology

The Viral/Molecular Core (Core D) provides reagents, facilities, lab services, consultation & training in support of innovative, multidisciplinary and translational HIV/AIDS research on the Penn/CHOP/Wistar campus with the broad goal of advancing collaborative research in the pathogenesis, treatment, and prevention of HIV/AIDS, serving CFAR investigators from multiple programs. The specific Core activities supporting this goal are to provide: ¿ Virology services including a repository of HIV-1 prototype, primary and mutant strains; virus isolation from patient samples; amplification of virus working stocks; structurally intact inactivated virions; HIV p24 and SIV p27 ELISA assay; and other viral services. ¿ Molecular services including support for realtime qPCR along with reagents, optimized primer-probe sets and custom primer/probe development; viral genotyping or cloning from clinical specimens; molecular virology support for translational research programs; and in the next cycle a new high density pyrosequencing service. ¿ Education, training, consultation and leadership in molecular virology activities including training in BSL3 practices for CFAR members lab personnel; training and consultation in virological and molecular techniques for CFAR investigator personnel; facilitating the dissemination and adoption of new technologies; and collaboration with and support of other Cores and campus programs in their HIV/AIDS research-related educational efforts. During the current funding cycle the Core provided support to 46 CFAR investigators, contributing to novel gene therapy approaches to HIV infection; better understanding of integration and establishment of latency; new approaches to vaccine design; advances in viral entry and resistance to entry inhibitors; effects of neuroimmune mediators & drugs of abuse; HIV neuropathogenesis; and other areas. The V/M Core has evolved significantly over the past 9 years in response to CFAR member needs and user feedback, levels of utilization, and internal & external advisory input, and collaborates closely with other CFAR Cores in support of these goals. The Core has been directed since its inception by Drs. Ronald Collman (director) and Luis Montaner (co-director) and, going forward, will be joined by Dr. F. (Rick) Bushman (co-director) to supervise the introduction and dissemination of new molecular sequencing technologies to CFAR investigators.

病毒/分子核心（核心D）提供试剂，设施，实验室服务，咨询和培训， 支持在Penn/CHOP/Wistar上进行创新、多学科和转化的艾滋病毒/艾滋病研究 校园的广泛目标是推进合作研究的发病机制，治疗， 预防艾滋病毒/艾滋病，为来自多个项目的CFAR调查人员提供服务。具体核心活动 支持这一目标的是： 病毒学服务，包括HIV-1原型、原代和突变株库;病毒分离 来自患者样本;病毒工作储备液的扩增;结构完整的灭活病毒体; HIV p24和 SIV p27 ELISA检测;和其他病毒服务。 沿着实时qPCR和试剂、优化引物探针等分子服务 套和定制引物/探针开发;从临床标本中进行病毒基因分型或克隆;分子 为转化研究计划提供病毒学支持;在下一个周期， 焦磷酸测序服务。 <$分子病毒学活动的教育、培训、咨询和领导，包括以下方面的培训： CFAR成员实验室人员的BSL 3实践;病毒学和分子生物学方面的培训和咨询 CFAR调查人员的技术;促进新技术的传播和采用; 与其他核心和校园计划在艾滋病毒/艾滋病研究相关的合作和支持 教育努力。 在本供资周期内，核心小组向46名CFAR调查员提供了支助， 艾滋病毒感染的新基因治疗方法;更好地理解整合和建立 潜伏期;疫苗设计的新方法;病毒进入和对进入抑制剂的抗性的进展;影响 神经免疫介质和药物滥用; HIV神经发病机制;和其他领域。V/M核心 在过去的9年里，为了响应CFAR成员的需求和用户的反馈， 利用，以及内部和外部咨询意见，并与其他CFAR核心密切合作，以支持 这些目标。核心自成立以来一直由罗纳德科尔曼博士（主任）和路易斯 Montaner（联合主任），并将继续与F。（Rick）Bushman（联合导演）监督 向CFAR调查人员介绍和传播新的分子测序技术。