SCHISTOSOMIASIS JAPONICA AND PARAGONIMIASIS--GENETIC DIVERSITY AND VACCINES

日本血吸虫病和肺吸虫病——遗传多样性和疫苗

• 批准号: 6100042
• 负责人: SHU-XIAN LIU
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF PARASITIC DISEASES
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 1999-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6100042
• 关键词: China; Primates; Schistosoma japonicum; Trematoda; Ungulate; animal population study; cysteine endopeptidases; disease vectors; dogs; gene therapy; genetic polymorphism; genetic strain; glutathione transferase; hamsters; helminthiasis; laboratory mouse; laboratory rat; recombinant DNA; restriction fragment length polymorphism; species difference; synthetic vaccines; systematic biology

Trematode infections with either the Schistosoma japonicum complex or paragonimus spp. are among the most important public health threats in the highly populated rural areas of the Yangtze River Basin of China. We propose to apply modern and innovative biotechnology towards the study and control of these two trematodiases. For S. japonicum we propose to develop two types of molecular vaccines using either recombinant polypeptides or plasmid (naked) DNA. A number of candidate cDNAs and/or their expressed polypeptides will be examined including a particularly promising S. japonicum glutathione-S-transferase (GST). In experimental animal models with mice, pigs and water buffaloes we have found that recombinant GST functions as an effective anti-disease vaccine for schistosomiasis japonica; because water buffalo are important animal reservoirs for this infection in humans we will evaluate GST and other vaccine candidates for their ability to interrupt transmission by reservoir host immunization. The second part of the S. japonicum project will rely on collecting molecular genetic data, which is specific for localized geographic Chinese strains. Using RAPD, rRNA gene RFLP, and single gene copy sequence methodologies we propose to uncover infraspecific genetic diversity (or cryptic species) with S. japonicum populations prevalent in the Yangtze River Basin. We will look for the emergence or reemergence of new Chinese schistosome species and determine the impact of sequence diversity on recombinant vaccine development. Approaches to paragonimiasis will follow similar lines (molecular vaccines and genetic diversity), but because of a paucity of existing taxonomic information we will first establish a sound taxonomic framework for the genus paragonimus in China with emphasis in this proposal on P westermani hypothesized species complex and the hypothesized p. skrjabini species complex. The molecular diversity data will be used to evaluate molecular vaccine candidates for paragonimiasis including a GST and cysteinyl protease.

日本血吸虫复合体的吸虫感染