CORE--MOLECULAR GENETICS SYSTEMATIC MEDICAL MALACOLOGY AND PARASITOLOGY

这一核心设施将为所有三个项目提供一个中心， 在分子生物学和生物技术的卓越研究所 寄生虫病（IPD）。 通过公共空间，大型设备， 在应用各种分子工具方面训练有素的专业知识， 加强分子系统医学软体动物学和寄生虫学 在警局有一个组件 拟议的核心将成为总理 分子寄生虫学和寄生虫生物技术资源中心 中国 具体而言，我们建议：1）研究分子遗传学和分歧， 利用现代技术对寄生虫和蜗牛宿主的目标种群进行监测 分子生物学和生物技术。基因分化将是 通过使用等位酶的遗传标记分析进行检查， 限制性片段长度多态性（RFLPs）和DNA测序， 目标基因这里的重点将是揭示遗传的程度， 寄生虫与其螺类种群间的趋同与共育 以前被认为是由同一物种组成的载体， 2）对遗传多样性进行分类 在三个蠕虫集团和他们的蜗牛载体通过 基因组和cDNA文库的构建; 3）提供计算机- 介导的分析能力，以评估分子分类学数据 收集在具体目标1和2; 4）开发新的分子 用于靶向疫苗递送的转染系统。

This core facility will serve all three projects by providing a center of excellence in molecular biology and biotechnology for the Institute of Parasitic Diseases (IPD). Through common space, large equipment, and trained expertise in applying diverse molecular tools we will greatly augment the molecular systematic medical malacology and parasitology component at the I.P.D. The proposed Core will become the premier Molecular Parasitology and Parasite Biotechnology resource center for all China. Specifically we propose: 1) To study molecular genetics and divergence of targeted populations of parasites and snail hosts using modern techniques in molecular biology and biotechnology. Genetic divergence will be examined through the analysis of genetic markers using allozymes, restriction fragment length polymorphisms (RFLPs), and DNA sequencing of targeted genes. The focus here will be to uncover the degree of genetic convergence and phylogeny among populations of parasites and their snail vectors previously thought to comprise the same species, and by implication, genetically uniform; 2) To catalogue the genetic diversity within the three helminth groups and their snail vectors through the construction of genomic and cDNA libraries; 3) To provide the computer- mediated analytic capabilities to assess the molecular taxonomic data collected in specific aims 1 and 2; 4) To develop novel molecular transfection systems for targeted vaccine delivery.