CORE--MOLECULAR GENETICS SYSTEMATIC MEDICAL MALACOLOGY AND PARASITOLOGY

该核心设施将为所有三个项目提供 在分子生物学和生物技术方面的卓越表现 寄生虫病(IPD)。通过公共空间、大型设备和 在应用各种分子工具方面训练有素的专业知识，我们将大大 加强分子系统医学软化学和寄生虫学研究 I.P.D.的组成部分。拟议的核心将成为主要的 全民分子寄生虫学和寄生虫生物技术资源中心 中国。 具体地说，我们建议：1)研究分子遗传学和遗传分化。 利用现代技术确定寄生虫和钉螺宿主的目标种群 在分子生物学和生物技术方面。基因分化将是 通过使用等位酶对遗传标记的分析进行检查， 限制性片段长度多态(RFLP)和DNA测序 靶向基因。这里的重点将是揭示基因的程度 寄生虫及其钉螺种群的聚集性和系统发育 以前被认为包含相同物种的载体，并通过 暗示，遗传一致；2)对遗传多样性进行分类 在三个蠕虫群内和他们的蜗牛媒介通过 基因组文库和cDNA文库的构建；3)提供计算机- 评估分子分类数据的中介分析能力 在特定目标1和2中收集；4)开发新的分子 靶向疫苗输送的转导系统。

This core facility will serve all three projects by providing a center of excellence in molecular biology and biotechnology for the Institute of Parasitic Diseases (IPD). Through common space, large equipment, and trained expertise in applying diverse molecular tools we will greatly augment the molecular systematic medical malacology and parasitology component at the I.P.D. The proposed Core will become the premier Molecular Parasitology and Parasite Biotechnology resource center for all China. Specifically we propose: 1) To study molecular genetics and divergence of targeted populations of parasites and snail hosts using modern techniques in molecular biology and biotechnology. Genetic divergence will be examined through the analysis of genetic markers using allozymes, restriction fragment length polymorphisms (RFLPs), and DNA sequencing of targeted genes. The focus here will be to uncover the degree of genetic convergence and phylogeny among populations of parasites and their snail vectors previously thought to comprise the same species, and by implication, genetically uniform; 2) To catalogue the genetic diversity within the three helminth groups and their snail vectors through the construction of genomic and cDNA libraries; 3) To provide the computer- mediated analytic capabilities to assess the molecular taxonomic data collected in specific aims 1 and 2; 4) To develop novel molecular transfection systems for targeted vaccine delivery.