CORE--DNA SEQUENCING FACILITY

核心——DNA测序设备

• 批准号: 6101642
• 负责人: ROBERT HASELKORN
• 金额: $ 10.11万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6101642
• 关键词: DNA; biomedical facility; nucleic acid sequence

The University of Chicago Cancer Research Center DNA Sequencing Facility provides automated DNA sequencing services. The goals of the Facility are to provide accurate sequences at the lowest cost and the fastest turn- around time possible. The Facility includes an ABl 377 automated sequenator, 92-well and 48-well thermal cyclers, a Macintosh computer, a high resolution printer, and several software packages for sequence editing and alignment, including the latest version of Sequencer form Gene Codes. Users obtain order forms from the Facility World Wide Web page, fill them out (including the names and descriptions of each DNA template to be sequenced), place them in a personal file at the Facility, and then deliver a hard copy of the order together with the DNA samples. Facility technicians run the cycle sequencing reactions, usually dye- terminator reactions with primers supplied by the users, and then the sequencing gels, each with 36 lanes. The resulting chromatograms are placed in each user file, from which they can be down-loaded for editing and alignment. Turn-around time is 3-4 days. Routine results give good sequence for 600 bases; some templates can be read beyond 800. Facility staff provide users with advice on cloning vectors, template preparation, and planning of sequencing projects.

芝加哥大学癌症研究中心 DNA 测序设施 提供自动化 DNA 测序服务。该设施的目标是 以最低的成本和最快的周转提供准确的序列 大约时间可能。该设施包括一台 ABl 377 自动 测序仪、92 孔和 48 孔热循环仪、Macintosh 计算机、 高分辨率打印机和多个用于序列的软件包 编辑和对齐，包括最新版本的 Sequencer 表单 基因密码。用户从设施万维网获取订单 页面，填写它们（包括每个 DNA 的名称和描述） 待排序的模板），将它们放入设施的个人文件中， 然后将订单的纸质副本与 DNA 样本一起交付。 设施技术人员运行循环测序反应，通常是染料- 与用户提供的引物进行终止反应，然后 测序凝胶，每个有 36 个泳道。所得色谱图为 放置在每个用户文件中，可以从中下载它们进行编辑 和对齐。周转时间为3-4天。常规结果良好 600个碱基的序列；一些模板可以读取超过800个。 设施 工作人员为用户提供有关克隆载体、模板制备、 和测序项目的规划。