CORE--AMES ASSAY

该计划项目的核心设施将存储、供应和运行 杂环胺类化学品的质量控制。这些化学品将会是 可从手头的库存、商业来源或将 根据需要合成，在LLNL或瑞典大学 农业科学。放射性标记化合物也将从 现货或综合股。重中之重是~3H标记的PhlP和PhlP 代谢物。 核心将提供后生杂环的质谱分析 来自食品的胺和诱变剂/致癌物形成模型反应， 杂环胺的代谢物，某些DNA和蛋白质加合物 与体内和体外代谢相关的实验和 剂量学项目。核心还将提供气相色谱-质谱仪 化学项目食品提取物的光谱分析。 食品和各种实验样本将使用 艾姆斯/沙门氏菌检测。致突变检测将在化学物质之前进行。 分析。合成产品、代谢物和诱变剂形成反应 还将进行突变活性测试，以支持一些 项目。

The Core Facility for this Program Project will store, supply and run quality control on the heterocyclic amine chemicals. The chemicals will be available from either stocks on hand, commercial sources, or will be synthesized as needed, here at LLNL or at the Swedish University of Agricultural Sciences. Radiolabeled compounds will also be supplied from current stocks or synthesized. Top priority is 3H labeled PHlP and PhlP metabolites. The core will provide mass spectrometry analysis of metagenic heterocyclic amines from foods and mutagen/carcinogen-forming model reactions, metabolites of the heterocyclic amines, some DNA- and protein-adducts from in vivo and in vitro experiments associated with the Metabolism and Dosimetry projects. The core will also provide gas chromatography-mass spectrometry analysis of food extracts for the Chemistry Project. Foods and various experimental samples will be tested using the Ames/Salmonella test. Mutagenic testing will be done prior to chemical analysis. Synthetic products, metabolites and mutagen-forming reactions will also be tested for mutagenic activity in support of a number of the Projects.