CORE--AMES ASSAY

本计划项目的核心设施将储存、供应和运行 杂环胺类化学品的质量控制。 这些化学物质将 可从手头的库存、商业来源获得，或将 根据需要合成，在LLNL或瑞典大学 农业科学。 放射性标记化合物也将由 当前库存或合成。 最高优先级是3 H标记的PHIP和PhIP 代谢物。 核心将提供元基因杂环的质谱分析 食物中的胺和诱变剂/致癌物形成模型反应， 杂环胺的代谢物，一些DNA和蛋白质加合物， 与代谢相关的体内和体外实验， 剂量测定项目。 核心还将提供气相色谱-质谱 化学项目食品提取物的光谱分析。 食品和各种实验样品将使用 艾姆斯/沙门氏菌试验。 将在化学品之前进行诱变试验 分析. 合成产物、代谢产物和诱变剂形成反应 还将进行致突变活性试验，以支持 项目

The Core Facility for this Program Project will store, supply and run quality control on the heterocyclic amine chemicals. The chemicals will be available from either stocks on hand, commercial sources, or will be synthesized as needed, here at LLNL or at the Swedish University of Agricultural Sciences. Radiolabeled compounds will also be supplied from current stocks or synthesized. Top priority is 3H labeled PHlP and PhlP metabolites. The core will provide mass spectrometry analysis of metagenic heterocyclic amines from foods and mutagen/carcinogen-forming model reactions, metabolites of the heterocyclic amines, some DNA- and protein-adducts from in vivo and in vitro experiments associated with the Metabolism and Dosimetry projects. The core will also provide gas chromatography-mass spectrometry analysis of food extracts for the Chemistry Project. Foods and various experimental samples will be tested using the Ames/Salmonella test. Mutagenic testing will be done prior to chemical analysis. Synthetic products, metabolites and mutagen-forming reactions will also be tested for mutagenic activity in support of a number of the Projects.