ANALYTICAL CHEMISTRY

这个项目的目的是调查美国人对熟食的饮食。 增加人体对杂环胺的摄入量 诱变剂/致癌物。我们将使用两种方法1)一种高效液相分析方法 已经在我们使用固相萃取的实验室中使用，以及2) 需要分析物衍生化的更灵敏的GC/MS方法 对于丰度较低的化合物的定量将更加有用。 环境中杂环胺致癌物质的十亿分之几的水平 食品需要广泛的样品准备和灵敏和特异 分析方法。杂环胺的数量需要方法 这将在每次分析中检测和量化几种化合物，这两种 提出的方法将满足这一要求。我们将与 由风险分析小组对本项目进行量化确定 人类饮食中的杂环胺含量。风险小组将 根据消耗率确定潜在的重要食物， 这个项目将量化诱变剂/致癌物的风险 计算。 我们还将研究模型反应中的诱变剂形成，以完成 一些未知和部分表征的杂环胺的鉴定 在熟食中。已识别的结构将在食品中进行量化，并将 可用于评价它们的生物学特性。我们会 探索减少杂环胺形成的实用方法 通过在烹饪前去除或降解前体而产生的致突变物/致癌物 对在此之后残留的特定化合物进行定量 程序。

The purpose of this project is to survey the American diet for cooked foods contributing to the human intake of heterocyclic amine mutagens/carcinogens. We will use two methods 1) an HPLC analysis method already in use in our laboratory which uses solid phase extractions, and 2) a more sensitive GC/MS method which requires derivitization of the analyte and will be more useful for quantitation of the less abundant compounds. The part-per-billion levels of the heterocyclic amine carcinogens in the foods requires extensive sample preparation and sensitive and specific analytical methods. The number of heterocyclic amines requires methods that will detect and quantify several compounds per analysis, the two proposed methods will fulfill this requirement. We will work closely with the risk analysis group of this project to determine the quantitative amounts of heterocyclic amines in the human diet. The risk group will determine the potentially important foods based of the rate of consumption, and this project will quantify the mutagens/carcinogens for risk calculations. We will also study mutagen formation in model reactions to complete the identification of some unknown and partly-characterized heterocyclic amines in cooked foods. Identified structures will be quantified in foods and be available for evaluation of their biological properties. We will investigate practical methods to reduce the formation of heterocyclic amine mutagens/carcinogens by precursor removal or degradation before cooking and quantitation of the specific compounds remaining following these procedures.