ADHESION PROPERTIES OF PORPHYROMONAS GINGIVALIS FIMBRIAE
牙龈卟啉单胞菌菌毛的粘附特性
基本信息
- 批准号:6104751
- 负责人:
- 金额:$ 14.59万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1997
- 资助国家:美国
- 起止时间:1997-12-01 至 1999-11-30
- 项目状态:已结题
- 来源:
- 关键词:Bacteroides gingivalis SDS polyacrylamide gel electrophoresis bacterial antigens cell adhesion chemical binding chimeric proteins disease /disorder model epitope mapping gene expression genetic mapping host neoplasm interaction human subject hydroxyapatites laboratory mouse laboratory rabbit laboratory rat microorganism culture molecular cloning mutant nucleic acid sequence periodontium disorder pilus polymerase chain reaction protein purification radiotracer site directed mutagenesis southern blotting synthetic peptide tritium
项目摘要
Our studies with purified fimbrial proteins have demonstrated an
interaction with salivary-coated hydroxyapatite (sHAP) that involves the
carboxyl third of the major 43 kDa fimbrillin subunit. In contrast,
immunodominant regions appear to reside in the N-terminus of the fimbrillin
subunit. The principal goal of this study is to further define the
structure-function relationship of fimbriae and fimbrial-associated
adhesins of P. gingivalis. Molecular genetic approaches to studying the
fimbriae will be used to evaluate the genes specifying the P. gingivalis
fimbrial-associated adhesins. DNA sequences adjacent to the 5' and 3' ends
of the fimbrillin gene will be examined to determine whether the gene
encoding for fimbrillin is part of a larger polycistonic unit that also
contains other fimbrial-associated components. In order to further define
the sHAP binding domains of the fimbrillin monomer, we propose to introduce
site-directed mutations in the binding sites of fimbrillin domains
identified in our previous studies. The mutant genes will be expressed as
fusion proteins, purified, and tested in the in vitro system for adherence
to sHAP or salivary components in solution. We will also pursue the direct
approach of insertional inactivation of fimbrial gene and complementation
studies. Mutant genes will be inserted into shuttle vectors which will be
used to complement fimA gene deficient mutants generated in our laboratory.
We will expand our studies to other strains of P. gingivalis by comparing
the variable and constant epitopes of the 43 kDa fimbrillin subunit from
several strains. Completion of these studies will provide, at the
molecular level, an understanding of the antigenic heterogeneity observed
among the strains. Synthetic peptides of the variable regions will be used
to identify their role in attachment to salivary-coated HAP as well as in
immune reactivity of fimbriae. Thus, the studies outlined in this
subproject could provide important information needed for a rational
approach for immunization to modulate colonization of the oral cavity.
我们对纯化菌毛蛋白的研究已经证明了
项目成果
期刊论文数量(0)
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专利数量(0)
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ROBERT J GENCO其他文献
ROBERT J GENCO的其他文献
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{{ truncateString('ROBERT J GENCO', 18)}}的其他基金
ADHESION PROPERTIES OF PORPHYROMONAS GINGIVALIS FIMBRIAE
牙龈卟啉单胞菌菌毛的粘附特性
- 批准号:
6296261 - 财政年份:1996
- 资助金额:
$ 14.59万 - 项目类别:
ADHESION PROPERTIES OF PORPHYROMONAS GINGIVALIS FIMBRIAE
牙龈卟啉单胞菌菌毛的粘附特性
- 批准号:
6238421 - 财政年份:1996
- 资助金额:
$ 14.59万 - 项目类别:
ADHESION PROPERTIES OF PORPHYROMONAS GINGIVALIS FIMBRIAE
牙龈卟啉单胞菌菌毛的粘附特性
- 批准号:
3775842 - 财政年份:
- 资助金额:
$ 14.59万 - 项目类别:
EPITOPE MAPPING OF BLACK-PIGMENTED BACTEROIDES FIMBRIAE
黑色拟杆菌菌毛的表位作图
- 批准号:
3875388 - 财政年份:
- 资助金额:
$ 14.59万 - 项目类别:
EPITOPE MAPPING OF BLACK-PIGMENTED BACTEROIDES FIMBRIAE
黑色拟杆菌菌毛的表位作图
- 批准号:
3917293 - 财政年份:
- 资助金额:
$ 14.59万 - 项目类别:
EPITOPE MAPPING OF BLACK-PIGMENTED BACTEROIDES FIMBRIAE
黑色拟杆菌菌毛的表位作图
- 批准号:
3940176 - 财政年份:
- 资助金额:
$ 14.59万 - 项目类别:
HOST PARASITE INTERACTIONS IN PERIODONTAL DISEASE
牙周疾病中宿主寄生虫的相互作用
- 批准号:
3951084 - 财政年份:
- 资助金额:
$ 14.59万 - 项目类别: