ADHESION PROPERTIES OF PORPHYROMONAS GINGIVALIS FIMBRIAE

牙龈卟啉单胞菌菌毛的粘附特性

基本信息

  • 批准号:
    6104751
  • 负责人:
  • 金额:
    $ 14.59万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1997
  • 资助国家:
    美国
  • 起止时间:
    1997-12-01 至 1999-11-30
  • 项目状态:
    已结题

项目摘要

Our studies with purified fimbrial proteins have demonstrated an interaction with salivary-coated hydroxyapatite (sHAP) that involves the carboxyl third of the major 43 kDa fimbrillin subunit. In contrast, immunodominant regions appear to reside in the N-terminus of the fimbrillin subunit. The principal goal of this study is to further define the structure-function relationship of fimbriae and fimbrial-associated adhesins of P. gingivalis. Molecular genetic approaches to studying the fimbriae will be used to evaluate the genes specifying the P. gingivalis fimbrial-associated adhesins. DNA sequences adjacent to the 5' and 3' ends of the fimbrillin gene will be examined to determine whether the gene encoding for fimbrillin is part of a larger polycistonic unit that also contains other fimbrial-associated components. In order to further define the sHAP binding domains of the fimbrillin monomer, we propose to introduce site-directed mutations in the binding sites of fimbrillin domains identified in our previous studies. The mutant genes will be expressed as fusion proteins, purified, and tested in the in vitro system for adherence to sHAP or salivary components in solution. We will also pursue the direct approach of insertional inactivation of fimbrial gene and complementation studies. Mutant genes will be inserted into shuttle vectors which will be used to complement fimA gene deficient mutants generated in our laboratory. We will expand our studies to other strains of P. gingivalis by comparing the variable and constant epitopes of the 43 kDa fimbrillin subunit from several strains. Completion of these studies will provide, at the molecular level, an understanding of the antigenic heterogeneity observed among the strains. Synthetic peptides of the variable regions will be used to identify their role in attachment to salivary-coated HAP as well as in immune reactivity of fimbriae. Thus, the studies outlined in this subproject could provide important information needed for a rational approach for immunization to modulate colonization of the oral cavity.
我们对纯化菌毛蛋白的研究已经证明了

项目成果

期刊论文数量(0)
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ROBERT J GENCO其他文献

ROBERT J GENCO的其他文献

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{{ truncateString('ROBERT J GENCO', 18)}}的其他基金

ADHESION PROPERTIES OF PORPHYROMONAS GINGIVALIS FIMBRIAE
牙龈卟啉单胞菌菌毛的粘附特性
  • 批准号:
    6296261
  • 财政年份:
    1996
  • 资助金额:
    $ 14.59万
  • 项目类别:
ADHESION PROPERTIES OF PORPHYROMONAS GINGIVALIS FIMBRIAE
牙龈卟啉单胞菌菌毛的粘附特性
  • 批准号:
    6238421
  • 财政年份:
    1996
  • 资助金额:
    $ 14.59万
  • 项目类别:
ADHESION PROPERTIES OF PORPHYROMONAS GINGIVALIS FIMBRIAE
牙龈卟啉单胞菌菌毛的粘附特性
  • 批准号:
    3775842
  • 财政年份:
  • 资助金额:
    $ 14.59万
  • 项目类别:
EPITOPE MAPPING OF BLACK-PIGMENTED BACTEROIDES FIMBRIAE
黑色拟杆菌菌毛的表位作图
  • 批准号:
    3875388
  • 财政年份:
  • 资助金额:
    $ 14.59万
  • 项目类别:
EPITOPE MAPPING OF BLACK-PIGMENTED BACTEROIDES FIMBRIAE
黑色拟杆菌菌毛的表位作图
  • 批准号:
    3917293
  • 财政年份:
  • 资助金额:
    $ 14.59万
  • 项目类别:
METABOLISM OF ORAL BACTERIA
口腔细菌的代谢
  • 批准号:
    3896733
  • 财政年份:
  • 资助金额:
    $ 14.59万
  • 项目类别:
METABOLISM OF ORAL BACTERIA
口腔细菌的代谢
  • 批准号:
    3939915
  • 财政年份:
  • 资助金额:
    $ 14.59万
  • 项目类别:
EPITOPE MAPPING OF BLACK-PIGMENTED BACTEROIDES FIMBRIAE
黑色拟杆菌菌毛的表位作图
  • 批准号:
    3940176
  • 财政年份:
  • 资助金额:
    $ 14.59万
  • 项目类别:
HOST PARASITE INTERACTIONS IN PERIODONTAL DISEASE
牙周疾病中宿主寄生虫的相互作用
  • 批准号:
    3951084
  • 财政年份:
  • 资助金额:
    $ 14.59万
  • 项目类别:
PERIODONTAL INFECTIONS
牙周感染
  • 批准号:
    3928686
  • 财政年份:
  • 资助金额:
    $ 14.59万
  • 项目类别:
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