METABOLISM OF ORAL BACTERIA
口腔细菌的代谢
基本信息
- 批准号:3939915
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:Actinomyces Streptococcus sanguis aerobic bacteria amylases anaerobic bacteria autoradiography dental research dentistry education gel filtration chromatography human subject membrane activity microorganism metabolism oral bacteria parotid gland postgraduate education radiotracer saliva submandibular gland
项目摘要
Research efforts have developed in two major areas;
1. Characterization of the effect of anaerobiosis on the
ultrastructure and surface proteins of gram-negative facultative
anaerobes. Findings suggest that anaerobiosis may play an
important role in the regulation of bacterial surface components.
The results of these studies have been recently published
(Infection and Immunity, 1987,55:2320-2323).
2. a. Characterization of the principal components forming
the salivary pellicle on gram-positive oral bacteria (Streptococci
and Actinomyces). Preliminary experiments involved the
incubation of pure cultures of various oral bacterial strains in
freshly collected human parotid or submandibular-sublingual
saliva, followed by the extraction of adsorbed salivary
components with 2%SDS. Salivary components bound to the
bacterial surface were identified by SDS-PAGE and
immunobloting. The principal salivary components eluted from
Streptococcus sanguis were the low molecular weight mucin
(MG2) and alpha-amylase, while MG2 was the principal component
eluted from other Streptococci and A. viscosus. The MG2 which
eluted from the surface of A. viscosus migrated with an
electrophoretic mobility similar to asialo-MG2. These
observations were verified by examining the interaction between
125I-HSMSL or 125I-HPS with several of the test strains. When
125I-salivary derived pellicles of S. sanguis were analyzed by SDS-
PAGE/autoradiography, the autoradiographs were dominated by a
component of approximately 55-60 kDa, as well as a low
molecular weight band. The salivary derived pellicles from the
other bacteria contained several other components of various
molecular weights. These data indicate that selective
interactions occur between the major salivary secretions and the
surface of the bacterial strains tested. A manuscript
summarizing these findings is in preparation.
b. Characterization of the binding of parotid amylase
(isoenzyme B1) to S. sanguis and other oral bacteria. Purified
amylase (isoenzyme B1) was prepared from parotid saliva by gel-
filtration chromatography, followed by FPLC anion-exchange
chromatography. Purity was assessed by electrophoresis and
amino-acid analysis. Experiments to determine the specificity of
binding of 125I-labelled amylase B1 to S. sanguis and other oral
bacteria are in progress.
在两个主要领域开展了研究工作;
1.厌氧对环境影响的表征
革兰氏阴性兼性肺炎的超微结构和表面蛋白
厌氧菌。研究结果表明,厌氧症可能会对
在调节细菌表面成分方面起着重要作用。
这些研究的结果最近发表了。
(感染与免疫,1987,55:2320-2323)。
2.a.形成的主成分的表征
口腔革兰氏阳性菌(链球菌)的唾液膜
和放线菌)。初步实验包括
不同口腔细菌株纯培养的体外培养
新鲜采集的人腮腺或下颌下-舌下
唾液,然后提取吸附的唾液
含有2%十二烷基硫酸钠的成分。唾液成分结合到
菌体表面用SDS-PAGE和
免疫印迹。洗脱出来的主要唾液成分
血链球菌是一种低分子粘蛋白
(Mg2)和α-淀粉酶,而Mg2是主成分
从其他链球菌和粘性酵母菌中洗脱出来。它的MG2
从粘性酵母菌表面洗脱出来的
电泳迁移率类似于asialo-Mg2。这些
观察结果被证实是通过检查
~(125)I-HSMSL或~(125)I-HPS与几株供试菌株结合。什么时候
用十二烷基硫酸钠-十二烷基硫酸钠对血链球菌~(125)I唾液液膜进行了分析。
PAGE/放射自显影术,放射自显影术主要由
大约55-60 kDa的成分,以及低
分子量带。唾液中的膜来自于
其他细菌含有多种不同种类的其他成分
分子量。这些数据表明,选择性
在主要的唾液分泌物和
被测试的细菌菌株的表面。一份手稿
总结这些发现正在准备之中。
B.腮腺淀粉酶的结合特性
(同工酶B1)对血链球菌和其他口腔细菌。纯净的
用凝胶法从腮腺唾液中提取淀粉酶(同工酶B1)。
过滤层析,然后FPLC阴离子交换
层析法。用电泳法和电泳法测定纯度
氨基酸分析。通过实验确定病毒的特异性
~(125)I标记的淀粉酶B1与血链球菌等的结合
细菌正在进化。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('ROBERT J GENCO', 18)}}的其他基金
ADHESION PROPERTIES OF PORPHYROMONAS GINGIVALIS FIMBRIAE
牙龈卟啉单胞菌菌毛的粘附特性
- 批准号:
6104751 - 财政年份:1997
- 资助金额:
-- - 项目类别:
ADHESION PROPERTIES OF PORPHYROMONAS GINGIVALIS FIMBRIAE
牙龈卟啉单胞菌菌毛的粘附特性
- 批准号:
6296261 - 财政年份:1996
- 资助金额:
-- - 项目类别:
ADHESION PROPERTIES OF PORPHYROMONAS GINGIVALIS FIMBRIAE
牙龈卟啉单胞菌菌毛的粘附特性
- 批准号:
6238421 - 财政年份:1996
- 资助金额:
-- - 项目类别:
ADHESION PROPERTIES OF PORPHYROMONAS GINGIVALIS FIMBRIAE
牙龈卟啉单胞菌菌毛的粘附特性
- 批准号:
3775842 - 财政年份:
- 资助金额:
-- - 项目类别:
ADHESION PROPERTIES OF PORPHYROMONAS GINGIVALIS FIMBRIAE
牙龈卟啉单胞菌菌毛的粘附特性
- 批准号:
3732532 - 财政年份:
- 资助金额:
-- - 项目类别: