CORE--ISLET ISOLATION AND TRANSPLANTATION FACILITY

核心——胰岛分离与移植设施

• 批准号: 6270861
• 负责人: JOHN Peter MORDES
• 金额: $ 8.04万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6270861
• 关键词: SCID mouse; biomedical facility; germ free condition; human tissue; laboratory mouse; laboratory rat; pancreatic islet transplantation; pancreatic islets; tissue /cell culture

The Islet Isolation and Transplantation Core will provide Project investigators with a reliable supply of isolated mouse, rat, and human pancreatic islets for use in transplantation. Mouse and rat islets will be prepared by the personnel of the Core. Human islets will be obtained from Dr. Camillo Ricordi at the University of Miami. The Islet Core will supervise the receipt, assessment, counting, and distribution of the human islets. Core personnel will also perform all islet transplantation surgery. The need for this Core was recognized in our previous proposal, and our laboratory has supported the activities of an Islet Isolation Core since 1991. The need for the core is determined by both biological and logistical considerations. Islets comprise less than 1% of the mass of the pancreas, are distributed nearly uniformly throughout a gland laden with digestive enzymes, and are fixed in position by a network of vasculature and fibrous tissue. Their isolation is a time-consuming procedure that requires considerable skill. On average, each mouse or rat islet transplant recipient requires all the islets that can be obtained from about four donors. In our experience, one technician can process enough donors to transplant 3 to 4 chemically diabetic mice per day. Each mouse requires 20 islets per gram of body weight to restore normoglycemia. Program Project investigators anticipate performing 1,500 to 2,000 transplants yearly, all using mouse recipients. One technician engaged in islet isolation will be required to satisfy the projected need for mouse and rat tissue and to transplant all mouse, rat, and human islets. The procedures required for islet transplantation beneath the kidney capsule require the development and maintenance of surgical skill. The level of skill required is particularly high because of the need to maintain strict asepsis; many of the transplants will be performed using immunodeficient scid mice. The proposed Islet Isolation Core facility is intended to avoid duplication of effort in the Program Project's laboratories and enhance quality control. The Core will also facilitate the Program Project concept by standardizing a key shared methodology, allowing comparisons of results among Projects. Our islet isolation procedure is a modified collagenase method carried out under aseptic conditions. If investigators should require additional processing of islets in the form of prolonged culture to remove passenger leukocytes or the preparation of porcine islets, the Islet Core can also provide this service. Islet transplantation will be performed by the Core staff in viral antibody free quarters in the case of rat recipients and in a special quarantine facility in the case of scid mice.

胰岛分离和移植核心将提供项目 研究人员提供了可靠的分离小鼠、大鼠和人 用于移植的胰岛。小鼠和大鼠的胰岛将 由核心人员准备的。人类胰岛将从 博士迈阿密大学的卡米洛·里科迪。核心岛将 监督人力资源的接收、评估、清点和分配 小岛核心人员也将进行所有胰岛移植 手术我们在以前的提案中确认了对这一核心的需要， 我们的实验室支持了胰岛隔离核心的活动， 自1991年以来。对核心的需要取决于生物学和 后勤考虑。 胰岛占胰腺质量的不到1%，分布在 几乎均匀地遍布充满消化酶的腺体， 通过脉管系统和纤维组织的网络固定在适当位置。他们的 分离是一个耗时的过程，需要相当多的技巧。 平均而言，每个小鼠或大鼠胰岛移植受体需要所有的 可以从大约四个供体获得的胰岛。根据我们的经验， 技术人员可以处理足够的捐赠者，以化学方式移植3到4个器官。 糖尿病小鼠每天。每只老鼠每克身体需要20个胰岛 体重恢复正常。项目研究人员预计， 每年进行1,500到2,000例移植手术，全部使用小鼠受体。 将需要一名从事胰岛分离的技术人员， 预计需要小鼠和大鼠组织，并移植所有小鼠，大鼠， 和人类的小岛 肾下胰岛移植所需的程序 胶囊需要发展和维持手术技巧。的 所需的技能水平特别高，因为需要 保持严格的无菌;许多移植手术将使用 免疫缺陷SCID小鼠拟建的Islet Isolation Core设施 旨在避免重复的努力，在该计划项目的 实验室，加强质量控制。核心还将促进 通过标准化关键共享方法， 可以比较项目之间的结果。 我们的胰岛分离程序是一种改良的胶原酶方法， 在无菌条件下。如果研究者需要额外的 以延长培养的形式处理胰岛以除去乘客 白细胞或猪胰岛的制备，胰岛核心也可以 提供这项服务。胰岛移植将由核心 工作人员在无病毒抗体宿舍的情况下，大鼠受体和 一个特殊的检疫设施在SCID小鼠的情况下。