CORE--ISLET ISOLATION AND TRANSPLANTATION FACILITY

核心——胰岛分离与移植设施

• 批准号: 6239300
• 负责人: JOHN Peter MORDES
• 金额: $ 10.61万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6239300
• 关键词: SCID mouse; biomedical facility; germ free condition; human tissue; laboratory mouse; laboratory rat; pancreatic islet transplantation; pancreatic islets; tissue /cell culture

The Islet Isolation and Transplantation Core will provide Project investigators with a reliable supply of isolated mouse, rat, and human pancreatic islets for use in transplantation. Mouse and rat islets will be prepared by the personnel of the Core. Human islets will be obtained from Dr. Camillo Ricordi at the University of Miami. The Islet Core will supervise the receipt, assessment, counting, and distribution of the human islets. Core personnel will also perform all islet transplantation surgery. The need for this Core was recognized in our previous proposal, and our laboratory has supported the activities of an Islet Isolation Core since 1991. The need for the core is determined by both biological and logistical considerations. Islets comprise less than 1% of the mass of the pancreas, are distributed nearly uniformly throughout a gland laden with digestive enzymes, and are fixed in position by a network of vasculature and fibrous tissue. Their isolation is a time-consuming procedure that requires considerable skill. On average, each mouse or rat islet transplant recipient requires all the islets that can be obtained from about four donors. In our experience, one technician can process enough donors to transplant 3 to 4 chemically diabetic mice per day. Each mouse requires 20 islets per gram of body weight to restore normoglycemia. Program Project investigators anticipate performing 1,500 to 2,000 transplants yearly, all using mouse recipients. One technician engaged in islet isolation will be required to satisfy the projected need for mouse and rat tissue and to transplant all mouse, rat, and human islets. The procedures required for islet transplantation beneath the kidney capsule require the development and maintenance of surgical skill. The level of skill required is particularly high because of the need to maintain strict asepsis; many of the transplants will be performed using immunodeficient scid mice. The proposed Islet Isolation Core facility is intended to avoid duplication of effort in the Program Project's laboratories and enhance quality control. The Core will also facilitate the Program Project concept by standardizing a key shared methodology, allowing comparisons of results among Projects. Our islet isolation procedure is a modified collagenase method carried out under aseptic conditions. If investigators should require additional processing of islets in the form of prolonged culture to remove passenger leukocytes or the preparation of porcine islets, the Islet Core can also provide this service. Islet transplantation will be performed by the Core staff in viral antibody free quarters in the case of rat recipients and in a special quarantine facility in the case of scid mice.

胰岛分离和移植核心将提供项目 调查人员提供可靠的隔离小鼠、大鼠和人 用于移植的胰岛。小鼠和大鼠的胰岛将是 由核心的人员准备。人类的胰岛将从 迈阿密大学的卡米洛·里科迪博士。小岛核心将 监督人员的接收、评估、清点和分发 小岛。核心人员还将进行所有胰岛移植 做手术。我们在以前的提案中承认了对这一核心的需求， 我们的实验室已经支持了一个小岛隔离核心的活动 自1991年以来。对核心的需求由生物和生物两方面决定 后勤方面的考虑。 胰岛分布在胰腺质量的1%以下 几乎均匀地贯穿整个腺体，充满消化酶，并且是 由血管和纤维组织组成的网络固定在适当的位置。他们的 隔离是一个耗时的过程，需要相当多的技能。 平均而言，每个小鼠或大鼠胰岛移植接受者需要所有的 可以从大约四个捐赠者那里获得的胰岛。在我们的经验中，有一个 技术人员可以处理足够的捐赠者来进行3到4次化学移植 糖尿病小鼠每天。每只老鼠每克身体需要20个胰岛 体重恢复正常血糖。计划项目调查人员预计 每年进行1500到2000次移植，全部使用小鼠接受者。 一名从事胰岛隔离的技术人员将被要求满足 预计需要小鼠和大鼠组织，并移植所有小鼠，大鼠， 和人类的小岛。 肾下胰岛移植所需的操作 胶囊需要发展和维护手术技巧。这个 所需的技能水平特别高，因为需要 保持严格的无菌；许多移植手术将使用 免疫缺陷的SCID小鼠。建议的小岛隔离核心设施是 旨在避免计划项目中的重复工作 实验室和加强质量控制。核心还将促进 计划项目概念通过标准化关键共享方法， 允许比较不同项目的结果。 我们的胰岛分离程序是一种改进的胶原酶方法 在无菌条件下。如果调查人员应该要求额外的 以长时间培养的形式加工胰岛以移走乘客 白血球或猪胰岛的制备，胰岛核也可以 提供这项服务。胰岛移植将由核心进行 工作人员在无病毒抗体宿舍的情况下，老鼠受体和 针对SCID小鼠的特殊检疫设施。